Expression of γ-H2AX, 53BP1 and Micronuclei as Genome Damage Biomarker of Population in Keang and Salumati Village, Mamuju West Sulawesi Province

IF 0.6 Q4 NUCLEAR SCIENCE & TECHNOLOGY

Atom Indonesia Pub Date : 2019-08-26 DOI:10.17146/aij.2019.847

A. D. Pratiwi, I.K.H. Basri, S. Purnami, M. Syaifudin, D. Tetriana, D. Yusuf, Tur Rahardjo, S. Nurhayati, N. Rahajeng, Y. Anggraito, I. Kashiwakura

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Abstract

The residents living in high background radiation area ha ve risk to be exposed by ionizing radiation that also potential ly cause their DNA damage. The aim of this study was to determine the expression of γ - H2AX , 53BP1 foci and micronuclei in the resident s who live in high background radiation area of Salumati village , Mamuju, West Sulawesi , Indonesia. Twenty one blood samples which consist of 11 from the study area and 10 from control were assessed for their expression of γ - H2AX and 53BPI foci by using specific antibodies and observed under fluorescence microscope whereas m icronuclei was detected after being cultured and giemsa stain ed according to standard procedure s . Results showed that both γ-H2AX and 53BP1 foci from high background area was lower than that of control area (0.37± 0.24 vs 0.19± 0.11 (p=0.03) for γ-H2AX and 0.61± 0.30 vs 0.31± 0.12 for 53BP1 (p=0.01)). The m ean of m icronuclei frequency in exposed area was 0.02 (0,01-0,03) while in control area was 0.02 (0.003-0.02). There w as statistical significant in corellation between both γ-H2AX , 53BP1 foci with micronuclei index in exposed area (p=0.02, p=0,04 respectively). In conclusion , there was a positive correlation between γ -H2AX and 53BP1 foci to micronuclei and this might be a clue of the occurrence of genome repairing mechanism caused by natural radiation at low dose chronical exposure in the studied area.

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γ-H2AX、53BP1和微核作为基因组损伤生物标志物在西苏拉威西马木居省Keang和Salumati村的表达

居住在高本底辐射地区的居民有受到电离辐射的风险，这也可能导致他们的DNA损伤。本研究旨在检测居住在印度尼西亚西苏拉威西马穆朱Salumati村高本底辐射区居民γ - H2AX、53BP1病灶和微核的表达。21份血样(研究区11份，对照组10份)采用特异性抗体检测γ - H2AX和53BPI病灶的表达，荧光显微镜下观察，培养后按标准程序进行吉氏染色，检测微核。结果表明，高背景区γ-H2AX和53BP1的焦度均低于对照区(γ-H2AX为0.37±0.24 vs 0.19±0.11 (p=0.03)， 53BP1为0.61±0.30 vs 0.31±0.12 (p=0.01))。暴露区m微核频率均值为0.02(0.01 ~ 0.03)，对照组为0.02(0.003 ~ 0.02)。γ-H2AX、53BP1病灶与暴露区微核指数相关性均有统计学意义(p=0.02、p= 0.04)。综上所述，γ -H2AX与53BP1微核病灶呈正相关，这可能是研究区域低剂量慢性自然辐射引起的基因组修复机制发生的线索。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Atom Indonesia NUCLEAR SCIENCE & TECHNOLOGY-

CiteScore

1.00

自引率

0.00%

发文量

审稿时长

16 weeks

期刊介绍： The focus of Atom Indonesia is research and development in nuclear science and technology. The scope of this journal covers experimental and analytical research in nuclear science and technology. The topics include nuclear physics, reactor physics, radioactive waste, fuel element, radioisotopes, radiopharmacy, radiation, and neutron scattering, as well as their utilization in agriculture, industry, health, environment, energy, material science and technology, and related fields.