Phenotypic and Genotypic Study of Antibiotic Resistance among Escherichia coli Isolates from Human Urinary Infection Cases in Bojnord Province

Q4 Medicine
M. Amiri, H. Farzin, M. Jamshidian-Mojaver
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引用次数: 1

Abstract

Background and Objective: Bacterial agents are the most common causes of urinary infection with Escherichia coli as the major causative organism. Accordingly, the current study aimed to determine the antibiotic resistance among Escherichia coli isolates from human urinary infection cases. Materials and Methods: The current experimental study was carried out on 50 specimens of positive cultures with urinary tract infection referred to Imam Reza Hospital Laboratory in Bojnord, Iran. The resistance and susceptibility of the isolates were assessed using disc diffusion method. Moreover, the presence of tetA, blaTEM, Sul1, aac(3)-IV, and aadA1 gens was examined using molecular methods with specific primers. Results: Prevalence of antibiotic resistance to ampicillin, cotrimaxazole, levofloxacin, nalidixic acid, ciprofloxacin, gentamicin, and nitrofurantoin was measured at 84%, 60%, 60%,52%, 44%, 22%, and 6%, respectively. in total, 50 E. coli strains were isolated were examined to determine blaTEM, aac(3)-IV, tetA, Sul, and aadA1 genes using polymerase chain reaction(PCR). Based on the obtained results, the frequency of blaTEM, aac(3)-IV, tetA, Sul1, aac(3)-IV, and aadA1 genes was reported as 24%, 12%,10%, 8%, and 14%, respectively. Conclusion: Disk diffusion agar method can be used as a primary screening method to determine antibiotic susceptibility for Escherichia coli isolates separated from urinary tract infections. In addition, genotypic method can be implemented for the accurate evaluation of the resistance of the isolates.
博伊诺省人尿路感染大肠埃希菌耐药表型和基因型研究
背景与目的:细菌是泌尿系感染最常见的原因,大肠杆菌是主要的病原菌。因此,本研究旨在确定从人尿路感染病例中分离的大肠杆菌对抗生素的耐药性。材料和方法:目前的实验研究是在伊朗Bojnord伊玛目礼萨医院实验室转诊的50例尿路感染阳性培养标本中进行的。采用圆盘扩散法测定菌株的耐药性和药敏性。此外,利用特异引物的分子方法检测了tetA、blaTEM、Sul1、aac(3)-IV和aadA1等原的存在。结果:氨苄西林、复方新美唑、左氧氟沙星、纳利地酸、环丙沙星、庆大霉素和呋喃妥因的耐药率分别为84%、60%、60%、52%、44%、22%和6%。共分离50株大肠杆菌,采用聚合酶链反应(PCR)检测blaTEM、aac(3)-IV、tetA、Sul和aadA1基因。根据所得结果,blaTEM、aac(3)-IV、tetA、Sul1、aac(3)-IV和aadA1基因的频率分别为24%、12%、10%、8%和14%。结论:圆盘扩散琼脂法可作为尿路感染分离的大肠埃希菌药敏的初步筛选方法。此外,基因型法可以准确评估分离株的耐药性。
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来源期刊
CiteScore
0.30
自引率
0.00%
发文量
16
审稿时长
8 weeks
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