Development of beta-carotene-loaded poly(lactic acid)/hydroxyapatite core-shell nanoparticles for osteoblast differentiation

IF 2.2 4区材料科学 Q2 MATERIALS SCIENCE, CERAMICS

Journal of Asian Ceramic Societies Pub Date : 2022-09-23 DOI:10.1080/21870764.2022.2127262

S. Lee, Y. Sugimoto, Katsuya Kato, T. Miyajima, M. Sakurai, F. Nagata

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引用次数: 1

Abstract

ABSTRACT Beta-carotene (BC) is a nutrient in vegetables and a precursor of vitamin A. BC has been reported to have anticarcinogenic, antiaging, and antioxidation properties and prevents heart diseases. Recently, BC has gained significant attention due to stimulating effect on osteoblast differentiation. Poly(lactic acid)/hydroxyapatite (PLA/HAp) core-shell nanoparticles have been reported earlier with a load capacity of 250% for water-insoluble substances, using a surfactant-free emulsification method. In this work, PLA/HAp core-shell nanoparticles loaded with BC were prepared, and osteoblast differentiation behavior was evaluated. BC was successfully loaded into PLA/HAp core-shell nanoparticles with diameters of approximately 30 nm. BC/loaded PLA/HAp core-shell nanoparticles stimulated osteoblast differentiation by upregulating collagen type I, osteopontin, and osteocalcin expression. In addition, the gene expression levels of these osteoblasts were significantly larger than those stimulated by PLA/HAp core-shell nanoparticles without BC and cultured in a differential medium (with ascorbic acid and β-glycerophosphate). PLA/HAp core-shell nanoparticles showed satisfactory cytocompatibility because they were attached to the osteoblasts. Consequently, BC was effectively delivered to osteoblasts by nanoparticles. These results suggested that BC-loaded PLA/HAp core-shell nanoparticles could enhance bone formation. Graphical Abstract

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用于成骨细胞分化的β -胡萝卜素负载聚乳酸/羟基磷灰石核壳纳米颗粒的开发

β -胡萝卜素(BC)是蔬菜中的一种营养物质，是维生素a的前体。据报道，BC具有抗癌、抗衰老和抗氧化的特性，可以预防心脏病。近年来，BC因其对成骨细胞分化的刺激作用而备受关注。聚(乳酸)/羟基磷灰石(PLA/HAp)核壳纳米颗粒采用无表面活性剂的乳化方法，对水不溶性物质的负载能力为250%。本研究制备了载BC的聚乳酸/羟基磷灰石核壳纳米颗粒，并对其成骨细胞分化行为进行了评价。BC成功加载到直径约为30 nm的PLA/HAp核壳纳米颗粒中。BC/负载PLA/HAp核壳纳米颗粒通过上调I型胶原、骨桥蛋白和骨钙素的表达来刺激成骨细胞分化。此外，这些成骨细胞的基因表达水平显著高于不含BC的PLA/HAp核壳纳米颗粒和在差异培养基(含抗坏血酸和β-甘油磷酸盐)中培养的成骨细胞。聚乳酸/羟基磷灰石核壳纳米颗粒与成骨细胞相结合，具有良好的细胞相容性。因此，BC通过纳米颗粒有效地递送到成骨细胞。这些结果表明，bc负载的PLA/HAp核壳纳米颗粒可以促进骨的形成。图形抽象

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来源期刊

Journal of Asian Ceramic Societies Materials Science-Ceramics and Composites

CiteScore

5.00

自引率

4.30%

发文量

审稿时长

10 weeks

期刊介绍： The Journal of Asian Ceramic Societies is an open access journal publishing papers documenting original research and reviews covering all aspects of science and technology of Ceramics, Glasses, Composites, and related materials. These papers include experimental and theoretical aspects emphasizing basic science, processing, microstructure, characteristics, and functionality of ceramic materials. The journal publishes high quality full papers, letters for rapid publication, and in-depth review articles. All papers are subjected to a fair peer-review process.