Topical mitomycin-C reduced number of myofibroblasts in healing anoplasty wounds in Wistar rats

Q1 Medicine

Wound Medicine Pub Date : 2019-06-01 DOI:10.1016/j.wndm.2019.100154

Harsali Fransiscus Lampus , Suryani As’ad , Rahmawati Minhajat , Warsinggih , Mochammad Hatta , Rina Masadah , Akhmad Makhmudi , Rosdiana Natzir , Wardihan Sinrang , Fachruddin Benyamin , Agussalim Bukhari , Nathania Wonoputri , Nita Mariana , Tommy Rubiyanto Habar

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引用次数: 1

Abstract

Background

: Mitomycin-C (MMC) has been used for treating stricture. Topical MMC has been proven to inhibit fibroblasts proliferation and reduce the number of fibroblasts. Myofibroblasts are originally from fibroblasts. The scarring potential of myofibroblasts has been documented in many anatomical sites. The purpose of this study is to analyze the effect of topical MMC on myofibroblasts in healing anoplasty wounds in Wistar rats.

Methods

: An anoplasty procedure was performed on Wistar rats (n=30) that were subsequently randomly placed into two equal groups; i.e., control and treated groups. In the treated group, topical mitomycin-C was applied to the wound following the anoplasty procedure. Five rats in each group were weekly sacrificed. The myofibroblasts in tissue samples were identified by immunohistochemical analysis of alpha-smooth muscle actin. The total of myofibroblasts was evaluated by scoring methods. The Mann-Whitney test was used to analyze the statistical significance of differences, and p values below 0.05 were considered to be significant.

Results

: The median myofibroblasts scores in the first week for the treated and control groups were 5 and 9, respectively. In the second and third week, the median myofibroblasts scores were 6 for both groups. Statistical evaluations revealed significant differences between the groups only in the first week (p=0.007).

Conclusions

: Topical MMC reduced the myofibroblasts in the first week of healing anoplasty wounds in Wistar rats.

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局部丝裂霉素c减少Wistar大鼠肛管成形术伤口愈合中肌成纤维细胞的数量

背景:丝裂霉素c (MMC)已被用于治疗狭窄。外用MMC已被证明可以抑制成纤维细胞的增殖并减少成纤维细胞的数量。肌成纤维细胞来源于成纤维细胞。肌成纤维细胞的瘢痕形成潜能已在许多解剖部位得到证实。本研究的目的是分析外用MMC对Wistar大鼠肛管成形术创面愈合过程中肌成纤维细胞的影响。方法:Wistar大鼠(n=30)行肛门成形术，随机分为两组;即对照组和治疗组。治疗组在肛管成形术后局部应用丝裂霉素c。每组每周处死5只大鼠。采用α -平滑肌肌动蛋白免疫组化方法鉴定组织样品中的肌成纤维细胞。采用评分法对肌成纤维细胞总数进行评估。采用Mann-Whitney检验分析差异的统计学意义，p值< 0.05为显著。结果:治疗组和对照组第一周肌成纤维细胞评分中位数分别为5分和9分。在第二和第三周，两组的肌成纤维细胞评分中位数均为6。统计学评价显示两组之间仅在第一周有显著差异(p=0.007)。结论:局部MMC在Wistar大鼠肛管成形术创面愈合的第一周内减少了肌成纤维细胞。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Wound Medicine Medicine-Surgery

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