Isolation and characterization of novel gene TaSSRP differentially expressed in wheat (Triticum aestivum L.) genotypes under heat stress

IF 1 4区生物学 Q3 PLANT SCIENCES

Indian Journal of Genetics and Plant Breeding Pub Date : 2022-04-25 DOI:10.31742/ijgpb.82.2.12

M. K. Azameti, Pankaj Singh, K. Gaikwad, M. Dalal, A. Arora, V. Rai, J. Padaria

引用次数: 0

Abstract

Analysis of heat stress-responsive transcriptome data in wheat genotype Raj 3765 revealed that expression of salt stress root protein (SSRP) RS1 was observed to increase by 8.29 folds in the flag leaf at the grain filling stage. Further validation of the gene under heat stress condition (42oC for 6 hours) by qRT-PCR revealed an increase of 8.42 folds. In-silico studies revealed that the gene is localized mainly in the cytoplasm, and did not contain transmembrane helix. The presence of 15 phosphorylation sites as indicated by in-silico analysis could play significant roles in the heat stress tolerance.

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热胁迫下小麦(Triticum aestivum L.)基因型差异表达新基因TaSSRP的分离与鉴定

对Raj 3765基因型小麦热胁迫响应转录组数据分析发现，籽粒灌浆期旗叶中盐胁迫根蛋白(SSRP) RS1的表达量增加了8.29倍。通过qRT-PCR进一步验证该基因在热应激条件下(42℃，6小时)的表达量增加了8.42倍。芯片研究表明，该基因主要定位于细胞质中，不含跨膜螺旋。硅分析表明，15个磷酸化位点的存在可能在耐热性中起重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Indian Journal of Genetics and Plant Breeding PLANT SCIENCES-

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Advance the cause of genetics and plant breeding and to encourage and promote study and research in these disciplines in the service of agriculture; to disseminate the knowledge of genetics and plant breeding; provide facilities for association and conference among students of genetics and plant breeding and for encouragement of close relationship between them and those in the related sciences; advocate policies in the interest of the nation in the field of genetics and plant breeding, and facilitate international cooperation in the field of genetics and plant breeding.