Effect of cryoprotectant type and equilibrium temperature on cryopreservation of alpaca sperm (Vicugna pacos)

Q4 Veterinary

Spermova Pub Date : 2022-07-31 DOI:10.18548/aspe/0010.03

W. Garcia, Veronica Macedo, Elizabeth Mendoza, Nubia Catacora, Julio Malaga

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引用次数: 0

Abstract

The objective of this study was to determine the effect of glycerol (GL), dimethyl sulfoxide (DMSO) and dimethylformamide (DMF) at temperatures of 5 °C and 16 °C of equilibrium on sperm quality, after cryopreservation. 6 males were used in the semen collection, motility parameters were evaluated with the CASA system, viability, acrosomal integrity and mitochondrial activity by flow cytometry in fresh and thawed semen. We collected 30 ejaculates of which 10 were discarded for not fulfilling (volume ≥ 1 ml and total motility ≥ 60 %), the 20 ejaculates were mesclo in a tube to form pool carrying out the assessment of the initial quality of fresh semen, obtaining a total motility (MT) 61,6 ± 3,4%, progressive motility (MP) 51,7 ± 3,7%, viability 78,1 ± 9.1%, mitochondrial activity (MA) 77,4 ± 7,7 and acrosomal integrity 88,9 ± 11,5%. After cryopreservation no equilibrium temperature effect was observed, independently of cryoprotectant in the parameters of seminal quality. Cryoprotective effect (P≤ 0.05) was observed, presenting superior values in the sperm conserved in GL with respect to the rest of cryoprotectants, with significant differences (P ≤ 0,05) in MT, MP, viability and AM and without significant differences in acrosomal integrity. The equilibrium temperature of 16 °C was effective for cryopreservation of alpaca sperm. In addition, the 5% GL preserved better the functional characteristics of sperm compared to 7% (DMF and DMSO).

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冷冻保护剂种类和平衡温度对羊驼精子冷冻保存的影响

本研究的目的是确定冷冻保存后，在5°C和16°C的平衡温度下，甘油（GL）、二甲基亚砜（DMSO）和二甲基甲酰胺（DMF）对精子质量的影响。采用6例男性精液采集，用CASA系统评价精液的运动参数，用流式细胞术评价新鲜精液和解冻精液的活力、顶体完整性和线粒体活性。我们收集了30支精液，其中10支因未完成而丢弃（体积≥1ml，总活力≥60%），20支精液在试管中制成池，对新鲜精液的初始质量进行评估，获得总活力（MT）61,6±3.4%，进行性活力（MP）51,7±3.7%，活力78,1±9.1%，线粒体活性（MA）为77.4±7,7，顶体完整性为88.9±11,5%。冷冻保存后，在精液质量参数上没有观察到与冷冻保护剂无关的平衡温度效应。观察到冷冻保护作用（P≤0.05），与其他冷冻保护剂相比，GL中保存的精子具有更高的价值，MT、MP、活力和AM存在显著差异（P≤0,05），顶体完整性无显著差异。16°C的平衡温度对羊驼精子的冷冻保存是有效的。此外，与7%（DMF和DMSO）相比，5%GL更好地保留了精子的功能特征。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Spermova Veterinary-Veterinary (miscellaneous)

CiteScore

0.30

自引率

0.00%

发文量

审稿时长

9 weeks