Identifying QTL for seed weight in a cross between vegetable and grain type soybeans

IF 1 4区生物学 Q3 PLANT SCIENCES

Indian Journal of Genetics and Plant Breeding Pub Date : 2020-11-30 DOI:10.31742/ijgpb.80.4.15

G. Khosla, B. S. Gill, A. Sirari, P. Sharma, I. Dhaliwal

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引用次数: 0

Abstract

The variation for 100 seed weight in F2 population derived from a cross, AGS456 (an exotic vegetable type from Taiwan)/SL958 of soybean followed a normal curve with a range of 8.00-27.22g indicating quantitative nature of genetic control for seed size. Parental lines were screened with 207 SSR markers to identify polymorphism and 90 primer pairs detected polymorphism between the parents. These ninety markers were used for detecting polymorphism between two extreme bulks for seed weight. Out of these, 18 primer pairs were polymorphic for the bulks and were used for bulk segregant analysis in 200 F2 plants. One major QTL for seed weight was identified on LG M with Sat_244 and Satt175 as flanking markers, explaining 19.0 per cent phenotypic variation. Two minor QTLs were also identified on LG D1b, one in interval Satt041-Sat_069 with an estimated phenotypic variation (R2) of 6.0 per cent and the other in interval Sat_069-Sat_0183 estimating 7.0 per cent phenotypic variance. The markers flanking TLs may help in marker-assisted selection (MAS) for improvement of seed weight in soybean after fine mapping and validation.

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菜型与粒型大豆杂交种子重QTL的鉴定

台湾外来蔬菜品种AGS456 /大豆品种SL958杂交F2群体百粒重的变异符合正态曲线，在8.00 ~ 27.22g范围内，说明遗传控制种子大小的数量性质。用207个SSR标记对亲本进行多态性筛选，90对引物检测到亲本间多态性。这90个标记用于检测种子重两个极端块间的多态性。其中18对引物具有整体多态性，可用于200株F2植株的整体分离分析。以sat244和sat175为侧翼标记，在LG M上鉴定出一个主要的种子重QTL，解释了19.0%的表型变异。在LG D1b上还鉴定出两个较小的qtl，一个位于区间Satt041-Sat_069，估计表型变异(R2)为6.0%，另一个位于区间Sat_069-Sat_0183，估计表型变异为7.0%。经过精细定位和验证，这些标记可能有助于大豆种子质量的标记辅助选择(MAS)。

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来源期刊

Indian Journal of Genetics and Plant Breeding PLANT SCIENCES-

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Advance the cause of genetics and plant breeding and to encourage and promote study and research in these disciplines in the service of agriculture; to disseminate the knowledge of genetics and plant breeding; provide facilities for association and conference among students of genetics and plant breeding and for encouragement of close relationship between them and those in the related sciences; advocate policies in the interest of the nation in the field of genetics and plant breeding, and facilitate international cooperation in the field of genetics and plant breeding.