Determinación de la calidad del semen criopreservado con lecitina de soya o yema de huevo, en machos cabríos

IF 0.3 Q4 VETERINARY SCIENCES

Abanico Veterinario Pub Date : 2021-12-01 DOI:10.21929/abavet2021.8

S. Moreno-Avalos, F. G. Véliz-Deras, Guadalupe Calderón-Leyva, Viridiana Contreras-Villarreal, J. M. Guillen-Muñoz, Oscar Ángel-García

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Abstract

The objective was to compare the quality of cryopreserved goat semen with soy lecithin or egg yolk. The semen was collected from male goats (n=4), two commercial diluents AndroMed® (1% soy lecithin, SL); Optidyl (20% (v/v) Tris-egg yolk; TY), and a citrate-egg yolk-based diluent (CY) were used in fresh semen (FS) and then cooled from 37 to 4 °C for 2 h (refrigerated semen, RS), afterwards straws were filled with semen and frozen in liquid nitrogen at -196 °C (FS). There were no differences (p>0.05) between diluents in the FS in the mass motility (MM; 4.7±0.26), sperm viability (SV; 74.1±1.66) and individual motility (MI; 62.3±4.0). In the same sense, for the RS there was no difference (p>0.05) between diluents with respect to MM (3.83±0.4) and MI (52.1±6.0), however, the SV varied (p<0.05) according to the diluent, observing the lowest viability in SL vs CY and TY (51.0±13.0 vs 71.3±3.0 and 69.0±3.1). Regarding FS the MM, MI and SV obtained better values (p<0.05) with the diluent TY vs SL and CY (2.4±0.5, 32.5±8.3, 41.3±13.0). The results showed a better cryopreservation of goat semen with the diluent Tris-yolk compared to that of soy lecithin.

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用大豆卵磷脂或蛋黄冷冻保存的公山羊精液质量的测定

目的是比较冷冻保存的山羊精液与大豆卵磷脂或蛋黄的质量。精液采集自雄性山羊（n=4），两种商业稀释剂AndroMed®（1%大豆卵磷脂，SL）；Optidyl（20%（v/v）Tris蛋黄；TY）和柠檬酸蛋黄基稀释剂（CY）用于新鲜精液（FS）中，然后从37℃冷却至4℃2小时（冷冻精液，RS），然后用精液填充吸管并在-196℃的液氮中冷冻（FS）。FS中稀释液在质量运动性（MM；4.7±0.26）、精子活力（SV；74.1±1.66）和个体运动性（MI；62.3±4.0）方面没有差异（p>0.05）。同样，RS中稀释液对MM（3.83±0.4）和MI（52.1±6.0）没有差异（p>0.05），但SV因稀释液而异（p<0.05），SL与CY和TY的存活力最低（51.0±13.0vs71.3±3.0和69.0±3.1）。在FS方面，稀释液TY与SL和CY的MM、MI和SV值更好（p<0.05）（2.4±0.5、32.5±8.3、41.3±13.0）。结果表明，稀释液Tris卵黄对山羊精液的冷冻保存效果优于大豆卵磷脂。

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Abanico Veterinario VETERINARY SCIENCES-

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