Producción de antígenos y estandarización de un ELISA casero indirecto para la detección de anticuerpos contra Anaplasma marginale

IF 0.7 4区农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Revista Mexicana de Ciencias Pecuarias Pub Date : 2022-09-09 DOI:10.22319/rmcp.v13i4.5976

Elizabeth Salinas Estrella, María Guadalupe Ortega Hernández, Erika Flores Pérez, Natividad Montenegro Cristino, Jesús Francisco Preciado de la Torre, Mayra Elizeth Cobaxin Cárdenas, Sergio D. Rodríguez Camarillo

引用次数: 0

Abstract

Serologic tests are important for the detection of specific antibodies against infectious agents. Commercial indirect ELISA are costly and usually as effective as in-house ELISAs. In the present work, it was prepared a batch of Anaplasma marginale crude antigen from infected blood, and tested it against official positive and negative serum controls and compared with an old batch of antigen. The new antigen batch showed an efficiency similar to the old batch. The sensitivity of the test was comparable between the new and old batches. Both, new and old antigen lots are being used at an excess. The new antigen lot is large enough to run thousands of tests at a more affordable price than commercial kits.

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检测边缘无浆体抗体的抗原生产和自制间接ELISA的标准化

血清学检测对于检测针对传染源的特异性抗体很重要。商业间接ELISA成本高昂，通常与内部ELISA一样有效。在本工作中，它从受感染的血液中制备了一批Anaplasma marginale粗抗原，并将其与官方阳性和阴性血清对照进行了测试，并与旧一批抗原进行了比较。新的抗原批次显示出与旧批次相似的效率。新批次和旧批次的检测灵敏度相当。新的和旧的抗原批次都被过量使用。新的抗原批次足够大，可以以比商业试剂盒更实惠的价格进行数千次检测。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Revista Mexicana de Ciencias Pecuarias AGRICULTURE, DAIRY & ANIMAL SCIENCE-

CiteScore

1.20

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The MEXICAN MAGAZINE OF SCIENCES PECUARIAS is an organ of scientific and technical diffusion of the livestock sector. Its periodicity is quarterly and arbitrated by pairs in the double-blind mode. Its objective is to make known the results of the research carried out by any scientific institution, in Mexico and in any part of the world, related to the livestock sciences, particularly those that refer to the different disciplines of Veterinary Medicine and Animal Science. The Journal is bilingual, publishes the complete articles in Spanish or English and is included in various indexing services and international dissemination platforms, such as the Index of Mexican Journals of Scientific and Technological Research of the National Council of Science and Technology (CONACYT); In the EBSCO Host database; In the Network of Scientific Journals of Latin America and the Caribbean, Spain and Portugal (RedALyC); In the Ibero-American Network of Scientific Journals of Free Access Veterinary Medicine. Indexed in the ISI Journal Citation Report Science Edition; And Elsevier''s SCOPUS and EMBASE indices.

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