Effect of Antioxidants on Cryopreserved Turbot (Scophthalmus maximus) Spermatozoa Quality and DNA Damage

IF 1.5 4区农林科学 Q3 FISHERIES

Turkish Journal of Fisheries and Aquatic Sciences Pub Date : 2022-10-04 DOI:10.4194/trjfas22300

H. Polat, İ. Kurtoğlu

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Abstract

The effects of several antioxidants added to cryopreservation extenders on turbot (Scophthalmus maximus) sperm post-thaw motility, fertilization, sperm characteristics, and DNA damage were investigated. The following antioxidants were used in conjunction with the conventional extenders: rosmarinic acid (RA) (25, 50, and 100 μg/ml); uric acid (UA) (25, 50, and 100 μg/ml); and taurine (TA) (25, 50, and 100 μg/ml). On sperm samples that had been diluted by the extenders at a 1:9 ratio, cryopreservation was carried out. The results demonstrated that the post-thaw motility of sperm was higher in 25 μg/ml RA (77.41±2.90%), followed by 50 μg/ml RA (74.86±3.2%), 50 and 100 μg/ml UA (69.56±3.30% and 70.74±2.51%, respectively) which significantly differentiated from conventional extender. The highest hatching rate (70.67±4.67%) was attained at UA 50 μg/ml, while the highest fertilization rate (81.67±2.52%) was observed in RA 25 μg/ml. At UA 50 μg/ml, the DNA fragmentation rate was lowest (5.07±1.57%). In conclusion, antioxidants had a protective impact on sperm of turbot that had been frozen and thawed.

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抗氧化剂对大菱鲆精子质量及DNA损伤的影响

研究了几种抗氧化剂对大菱鲆精子解冻后活力、受精、精子特性和DNA损伤的影响。以下抗氧化剂与常规填充剂一起使用：迷迭香酸（RA）（25、50和100μg/ml）；尿酸（UA）（25、50和100μg/ml）；牛磺酸（TA）（25、50和100μg/ml）。对用填充剂以1:9的比例稀释的精子样本进行冷冻保存。结果表明，25μg/ml RA的精子解冻后活力较高（77.41±2.90%），其次是50μg/ml RA（74.86±3.2%）、50μg/ml UA和100μg/ml UA（分别为69.56±3.30%和70.74±2.51%），与常规扩张剂有显著差异。UA 50μg/ml时孵化率最高（70.67±4.67%），RA 25μg/ml时受精率最高（81.67±2.52%）。在UA为50μg/ml时，DNA断裂率最低（5.07±1.57%）。

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来源期刊

Turkish Journal of Fisheries and Aquatic Sciences FISHERIES-MARINE & FRESHWATER BIOLOGY

CiteScore

3.10

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： Turkish Journal of Fisheries and Aquatic Sciences" (TrJFAS) is a refereed academic journal has been published by Central Fisheries Research Institute of Turkey and Japan International Cooperation Agency (JICA), and published in English. It aims to address research and needs of all working and studying within the many varied areas of fisheries and aquatic sciences. The Journal publishes English language original research papers, critical review articles, short communications and technical notes on applied or scientific research relevant to freshwater, brackish and marine environments. TrJFAS was published biannually (April & November) between 2001 and 2009. A great number of manuscripts have been submitted to the journal for review from acceptance of the SCI index. Thereby, the journal has been published quarterly (March, June, September and December) from 2010 to 2017. The journal will be published monthly in 2018.