Dietary Protein Replacement of Fish Meal with Black Soldier Fly Larvae Meal: Effects on Growth, Whole-Body Composition, Digestive Enzyme Activity, Muscle-Growth-Related Gene Expression and Haemato-Biochemical Responses of Juvenile Goldfish, Carassius auratus

IF 1.5 4区农林科学 Q3 FISHERIES

Turkish Journal of Fisheries and Aquatic Sciences Pub Date : 2022-09-22 DOI:10.4194/trjfas21837

A. Kamalii, Cheryl Antony, B. Ahilan, A. Uma, E. Prabu

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引用次数: 3

Abstract

A 60-day indoor growth trial was carried out to investigate the effects of dietary replacement of fish meal (FM) protein with black soldier fly (Hermetia illucens) larvae meal (BSFLM) at graded concentrations on bio-growth parameters, whole-body chemical composition, digestive enzyme activity, muscle-growth-related gene expression and haemato-biochemical responses of goldfish (Carassius auratus) juveniles. Six isonitrogenous and isoenergetic diets were formulated with BSFLM to substitute FM protein at 0 (T0), 20 (T20), 40 (T40), 60 (T60), and 80 (T80), and 100 % (T100). Each diet was randomly assigned to triplicate groups of 20 fish (Mean weight: 2.8±0.3 g) per aquaria were fed twice a day. Goldfish juveniles fed the T60 diet exhibited maximum growth and feed utilization. However, escalating the percentage of fishmeal substitution with BSFLM above 60 percent led to a significant reduction in growth and feed utilization. The protease activity and the haemoglobin (Hb) value were high in fishes fed with the T60 diet. The MyoD was upregulated in fish fed T40 and T60 diets, while the myostatin was downregulated in fish-fed T40 and T60 diets. Thus, the fishmeal protein substituted at 60% with BSFLM with an inclusion level of 201 g/kg of diet will be suitable for the goldfish juveniles.

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饲料中蛋白质替代鱼粉:对鲫鱼幼鱼生长、全身成分、消化酶活性、肌肉生长相关基因表达和血液生化反应的影响

进行了为期60天的室内生长试验，以研究日粮中以不同浓度的黑蝇幼虫粉（BSFLM）代替鱼粉（FM）蛋白对生物生长参数、全身化学成分、消化酶活性，金鱼幼鱼的肌肉生长相关基因表达和血液生化反应。在0（T0）、20（T20）、40（T40）、60（T60）、80（T80）和100%（T100）时，用BSFLM替代FM蛋白，配制了6种等氮和等能日粮。每种饮食被随机分为三组，每组20条鱼（平均重量：2.8±0.3克），每个水族馆每天喂食两次。喂食T60日粮的金鱼幼鱼表现出最大的生长和饲料利用率。然而，使用BSFLM将鱼粉替代率提高到60%以上，导致生长和饲料利用率显著降低。T60日粮喂养的鱼类蛋白酶活性和血红蛋白（Hb）值较高。MyoD在T40和T60日粮的鱼类中上调，而肌生长抑制素在T40或T60日料的鱼类中下调。因此，用BSFLM以60%取代的鱼粉蛋白，其包含水平为201g/kg日粮，将适合金鱼幼鱼。

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来源期刊

Turkish Journal of Fisheries and Aquatic Sciences FISHERIES-MARINE & FRESHWATER BIOLOGY

CiteScore

3.10

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： Turkish Journal of Fisheries and Aquatic Sciences" (TrJFAS) is a refereed academic journal has been published by Central Fisheries Research Institute of Turkey and Japan International Cooperation Agency (JICA), and published in English. It aims to address research and needs of all working and studying within the many varied areas of fisheries and aquatic sciences. The Journal publishes English language original research papers, critical review articles, short communications and technical notes on applied or scientific research relevant to freshwater, brackish and marine environments. TrJFAS was published biannually (April & November) between 2001 and 2009. A great number of manuscripts have been submitted to the journal for review from acceptance of the SCI index. Thereby, the journal has been published quarterly (March, June, September and December) from 2010 to 2017. The journal will be published monthly in 2018.