Role of heavy metals on antibiotic resistance properties in lactose fermenting isolates collected from textile industry effluents

Afroza Parvin Rokunozzaman, M. Hasan, P. Debnath, Md. Ashraful Hasan, Md. Mozammel Hossain, Mohammad Mahfuz Ali Khan Shawan, A. Halim, M. Rahman, Sabir Hossain, Sohel Ahmed
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Abstract

It is crucial to monitor the microbial ambience in heavy metal enriched industrial effluents that generally discharge into the environment. Therefore, the present study aimed to understand the load of heavy metals in multidrug-resistant lactose fermenting microorganisms isolating from textile industry effluents. Samples were collected from five different textile industrial canals. In most of the cases, the colony forming units (CFUs) per plate were uncountable at 10-6 dilution but countable at 10-10 dilution. A total of 100 lactose fermenting (LF) isolates were selected using 4 differential media and tested for the determination of minimum inhibitory concentrations (MICs) of heavy metals and antibiotic susceptibility. For nickel (Ni), the MIC was 0.3 mM for almost 98% isolates. The MIC was 0.5 mM for chromium (Cr) in almost 99% isolates. For lead (Pb), 100% isolates had a MIC of 0.4 mM. The most prevalent (36%) resistance pattern was found for sulfamethoxazole-trimethoprim (SXT) and only 10% isolates showed resistance to cefotaxime (CTX). The combined effect of heavy metals and antibiotics revealed that in most cases, the antibiotic zone of inhibition was increased. Plasmid profiling showed that among 14 selective isolates, high sized (21kb) plasmid was found in 6 isolates.   Key words: Antibiotics, effluents, heavy metals, minimum inhibitory concentration (MIC), antimicrobial resistance.
重金属对纺织工业废水中乳糖发酵分离物抗生素耐药性的影响
监测通常排放到环境中的富含重金属的工业废水中的微生物环境至关重要。因此,本研究旨在了解从纺织工业废水中分离出的耐多药乳糖发酵微生物中的重金属含量。从五条不同的纺织工业运河中采集样本。在大多数情况下,每个平板的菌落形成单位(CFU)在10-6稀释时是不可计数的,但在10-10稀释时是可计数的。使用4种不同的培养基选择总共100个乳糖发酵(LF)分离株,并测试重金属的最小抑制浓度(MIC)和抗生素敏感性。对于镍(Ni),几乎98%的分离物的MIC为0.3mM。在几乎99%的分离物中,铬(Cr)的MIC为0.5mM。对于铅(Pb),100%的分离株的MIC为0.4mM。对磺胺甲恶唑-甲氧苄啶(SXT)的耐药性最为普遍(36%),只有10%的分离株对头孢噻肟(CTX)表现出耐药性。重金属和抗生素的联合作用表明,在大多数情况下,抗生素的抑制区增加。质粒图谱分析表明,在14个选择性分离株中,6个分离株中发现了21kb的大质粒。关键词:抗生素,废水,重金属,最低抑菌浓度(MIC),抗微生物耐药性。
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21
审稿时长
3.8 months
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