A SNP at MicroRNA binding site of epidermal growth factor receptor 3́ untranslated region associated with Yangzhou geese egg production

Q4 Biochemistry, Genetics and Molecular Biology

Animal Gene Pub Date : 2022-03-01 DOI:10.1016/j.angen.2021.200123

Murtada Alsiddig , Tarig Badri , Hind Widaa , Bojiang Li , Yu Shigang , Jie Chen , Honglin Liu

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引用次数: 1

Abstract

Epidermal growth factor receptor (EGFR) gene plays a pivotal role in cell communication and proliferation in both vertebrates and invertebrates. The objectives of this study were to monitor for single nucleotide polymorphisms (SNPs) in the 3′untranslated region (3′-UTR) of EGFR in geese and evaluate the relationship between the polymorphisms of these SNPs and egg production. Using the direct sequencing method, a single nucleotide polymorphism c.*7750G > A was detected in 3′-UTR region of EGFR gene. Two alleles (G and A) and three genotypes (GG, AG and AA) were identified. Association analysis results showed that c.*7750G > A SNP was significantly associated with egg production at 34-week egg-laying period (P < 0.01). Geese with GG genotype (mutant) significantly produced more eggs compared to those with AA genotype (wildtype). Quantitative real-time PCR indicated that the expression levels of geese EGFR mRNA was highly expressed in kidney and granulosa cells and also expressed in follicles granulosa cells at different egg production stages with high expression in the large white follicle. Also, the mRNA expression level of EGFR in geese ovaries indicated that the geese with GG genotype recorded significantly higher expression levels compared to the geese with AA genotype. Luciferase activity assays showed that dme-miR-5-3p could significantly decrease luciferase activity in the presence of allele A compared with negative control and allele G (P < 0.05). These results suggest that c.*7750G > A in the 3′-UTR of EGFR gene may play a significant role in egg production in Yangzhou geese.

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扬州鹅产蛋相关表皮生长因子受体3非翻译区MicroRNA结合位点的SNP

表皮生长因子受体(Epidermal growth factor receptor, EGFR)基因在脊椎动物和无脊椎动物的细胞通讯和增殖中起着关键作用。本研究的目的是监测鹅EGFR 3 '非翻译区(3 ' -UTR)的单核苷酸多态性(SNPs)，并评价这些snp多态性与产蛋率的关系。采用直接测序法，单核苷酸多态性c.*7750G >在EGFR基因的3 ' -UTR区检测到A。鉴定出2个等位基因(G和A)和3个基因型(GG、AG和AA)。关联分析结果显示，c.*7750G >一个SNP与产蛋期34周的产蛋量显著相关(P <0.01)。GG基因型(突变型)鹅产蛋率显著高于AA基因型(野生型)鹅。实时荧光定量PCR结果显示，鹅EGFR mRNA在不同产蛋阶段的肾细胞和颗粒细胞中均有高表达，在卵泡颗粒细胞中也有表达，在白色大卵泡中表达量较高。EGFR mRNA在鹅卵巢中的表达水平也表明，GG基因型鹅的表达水平显著高于AA基因型鹅。荧光素酶活性测定显示，与阴性对照和等位基因G相比，在等位基因A存在时，dme-miR-5-3p能显著降低荧光素酶活性(P <0.05)。这些结果表明c.*7750G >EGFR基因3 ' -UTR上的A可能在扬州鹅产蛋过程中起重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Animal Gene Agricultural and Biological Sciences-Insect Science

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期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.