Antimicrobial activity of Helichrysum plicatum DC.

IF 0.8 4区工程技术 Q4 ENGINEERING, CHEMICAL

Hemijska Industrija Pub Date : 2017-09-25 DOI:10.2298/HEMIND160118044B

Dubravka Bigović, T. Stevic, T. Janković, Nebojša Noveski, D. Radanović, D. Pljevljakušić, Z. Djuric̀

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引用次数: 10

Abstract

Dry flower heads of Helichrysum plicatum were characterized by HPLC-DAD and a detailed antimicrobial assay of its ethanol extract was performed. Identification of phenolic compounds indicated the presence of apigenin, naringenin and kaempferol as free aglycones, glycosides of apigenin, naringenin, quercetin and kaempferol as well as chlorogenic acid and chalcone derivate. Antimicrobial activity of the extract was evaluated against various bacteria and fungi as well as yeast Candida albicans using microdilution method. Gram-positive bacteria were more sensitive to the tested extract ( MIC values were to 0.02 mg/mL) than Gram-negative bacteria (the greatest MIC was 0.055 mg/mL). Regarding pathogenic fungi, our tests demonstrated that fungi were more sensitive to the tested extract than bacteria. The growth of the majority of the tested fungi was inhibited by concentration of 0.005 mg/mL. Moreover, the extract was significantly more active than commercial fungicide, fluconazole. The results of our tests indicate that the extract of H. plicatum has significant antimicrobial activity and may find application in the pharmaceutical and food industry and organic agriculture.

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蜡菊的抗菌活性研究。

采用HPLC-DAD对蜡菊干花头进行了表征，并对蜡菊乙醇提取物进行了详细的抑菌试验。酚类化合物的鉴定表明，芹菜素、柚皮素和山奈酚为游离苷元，芹菜素、柚皮素、槲皮素和山奈酚的苷元，以及绿原酸和查尔酮衍生物。用微量稀释法测定了提取物对多种细菌、真菌及白色念珠菌的抑菌活性。革兰氏阳性菌对浸提物的MIC值为0.02 mg/mL，比革兰氏阴性菌更敏感(MIC值最高为0.055 mg/mL)。对于致病真菌，我们的试验表明，真菌比细菌对所测试的萃取物更敏感。0.005 mg/mL的浓度对大部分真菌的生长有抑制作用。此外，该提取物的活性明显高于市售杀菌剂氟康唑。实验结果表明，该提取物具有显著的抗菌活性，在医药、食品工业和有机农业等领域具有广阔的应用前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Hemijska Industrija 工程技术-工程：化工

CiteScore

1.60

自引率

11.10%

发文量

审稿时长

6-12 weeks

期刊介绍： The Journal Hemijska industrija (abbreviation Hem. Ind.) is publishing papers in the field of Chemical Engineering (Transport phenomena; Process Modeling, Simulation and Optimization; Thermodynamics; Separation Processes; Reactor Engineering; Electrochemical Engineering; Petrochemical Engineering), Biochemical Engineering (Bioreactors; Protein Engineering; Kinetics of Bioprocesses), Engineering of Materials (Polymers; Metal materials; Non-metal materials; Biomaterials), Environmental Engineeringand Applied Chemistry. The journal is published bimonthly by the Association of Chemical Engineers of Serbia (a member of EFCE - European Federation of Chemical Engineering). In addition to professional articles of importance to industry, scientific research papers are published, not only from our country but from all over the world. It also contains topics such as business news, science and technology news, information on new apparatus and equipment, and articles on environmental protection.