Germination, in vitro Propagation and Acclimatization in Lavandula Angustifolia

Abstract

This research focused on finding the best method for seed in vitro germination in Lavandula angustifolia and optimizing the medium for plant propagation. Seeds were sterilized and subjected to various treatments to break dormancy, then placed on half-strength MS (1/2MS) or distilled H2O + phytagel. Germination percentages were assessed and plantlets propagated on MS without growth regulators or with zeatin (0.5, 1, 2 mg/l), 1 mg/l BA + 0.5 mg/l IBA, 2 mg/l BA + 1 mg/l IBA or 3 mg/l BA + 1.5 mg/l IBA. After 8 weeks growth parameters were recorded and plants were acclimatized. Immersion in 20 mg/100 ml GA3 solution for 24 hours at 4°C was the most effective in breaking dormancy. Stratification at 4°C for 8 weeks and soaking in a solution of 0.5% H2O2 at 23°C for 24 hours also proved beneficial but to a smaller degree. Half-strength MS was the best germination medium. Shoot development was the highest in MS supplemented with zeatin (2 or 1 mg/l) while roots formed only in the control. Callus induction percentage was best in the presence of 3 mg/l BA + 1.5 mg/l IBA but decreasing concentrations increased callus weight. Plant acclimatization was more successful in moss:sand - 1:2 than in vermiculite:perlite:sand - 2:2:1.