Light condition, flask sealing, and cultivation time on the germination and initial in vitro development of Dendrobium nobile Lindl.

IF 1.1 Q3 HORTICULTURE

Ornamental Horticulture Pub Date : 2022-12-01 DOI:10.1590/2447-536x.v28i4.2515

Isabella de Souza Ribeiro, L. M. Ribeiro, J. S. Soares, J. Ramos, J. C. Sorgato

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引用次数: 1

Abstract

Abstract One of the obstacles for the production of Orchidaceae plants is the seed propagation. Thus, in in vitro cultivation, luminosity and the type of sealing of the bottles are factors that influence germination and initial development of orchids. Therefore, the objective was to evaluate the light condition, the type of sealing of the bottles and the evaluation time in germination and initial in vitro development of Dendrobium nobile Lindl. After sowing, cultures were subjected to two irradiance conditions according to the sealing of the flasks: 7.0 µmol m-2 s-1 (threaded cap) and 19.0 µmol m-2 s-1 (plastic film) and two light conditions: white fluorescent lamp and red fluorescent lamp. At 30, 60 and 90 days, the percentage of germination, survival, of protocorms in stage 1, stage 2, stage 3 and stage were evaluated 4. DIC was used, in a sub-divided plot scheme for 30 and 60 days after sowing and in a 2 x 2 factorial scheme at 90 days. The highest germination percentage was observed when using white fluorescent lamp with a plastic film cover (70.33%) and at 30 days (70.13%). The highest survival percentage (100%) was observed when using plastic film cover and white fluorescent lamp at 90 days. The greatest development of propagules, reaching stage 4 was verified in the red fluorescent lamp with threaded cap (9.55%).

查看原文本刊更多论文

光照条件、瓶封和培养时间对石斛萌发和离体发育的影响。

摘要兰科植物生产的障碍之一是种子繁殖问题。因此，在离体培养中，光照和瓶封类型是影响兰花萌发和初期发育的因素。因此，本研究的目的是评价石斛萌发和离体发育初期的光照条件、瓶封方式和评价时间。播种后，根据烧瓶的密封，将培养物置于两种辐照条件下:7.0µmol m-2 s-1(螺纹盖)和19.0µmol m-2 s-1(塑料薄膜)，两种光照条件:白色荧光灯和红色荧光灯。在30、60、90 d时，分别测定1、2、3、4期原球茎的发芽率和成活率。DIC在播种后30天和60天采用细分小区方案，90天采用2 × 2因子方案。白色荧光灯覆盖塑料薄膜时萌发率最高(70.33%)，30 d时萌发率最高(70.13%)。90 d时使用塑料薄膜覆盖和白色荧光灯时存活率最高(100%)。在带螺纹灯头的红色荧光灯下繁殖体发育最大，达到第4期(9.55%)。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Ornamental Horticulture Agricultural and Biological Sciences-Horticulture

CiteScore

2.20

自引率

14.30%

发文量

审稿时长

50 weeks

期刊介绍： The Journal ORNAMENTAL HORTICULTURE - OH (Revista Brasileira de Horticultura Ornamental) is an official publication of the Brazilian Society for Floriculture and Ornamental Plants (Sociedade Brasileira de Floricultura e Plantas Ornamentais) - SBFPO Short title of the journal: ORNAM. HORTIC. This Journal aim to publish technical and scientific researches in floriculture, ornamental plants, landscaping and landscapes from members of this Society and others. The manuscripts can be submitted in portuguese, but publication must be in English language (U.S.). All the manuscripts submitted to Ornamental Horticulture (Revista Brasileira de Horticultura Ornamental) - OH must not have been submitted or published at the same time, partial or totally, to other journal. It is allowed to read, download, copy, distribute, print, search, or link to parts or the full texts to use them for any other lawful purpose. The reproduction of articles published in this journal is authorized, under the condition that the source is acknowledged.