Comparing concentration of urinary inflammatory cytokines in interstitial cystitis, overactive bladder, urinary tract infection, and bladder cancer

IF 0.8 Q4 UROLOGY & NEPHROLOGY
M. Chancellor, L. Lamb, E. Ward, S. Bartolone, Alexander L. Carabulea, Prasun Sharma, Joseph J. Janicki, Christopher Smith, M. Laudano, N. Abraham, Bernadette M. Zwaans
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引用次数: 4

Abstract

Purpose: We sought to determine if urinary cytokine concentration profiles were different between various bladder conditions. Materials and Methods: Participants at three clinical sites completed a demographics survey and provided a urine sample in a collection cup containing a room-temperature urine preservative. Participants were divided into the following categories based on physician-documented diagnosis: asymptomatic control, nonulcerative interstitial cystitis (IC), overactive bladder with incontinence (OAB wet), urinary tract infection (UTI), and bladder cancer. Urinary cytokines were measured through Luminex multiplex assay. Results: Two hundred and seventy-seven urine samples were collected from three clinical sites. Urinary pro-inflammatory cytokines had an increasing trend in bladder disease versus control, with a significant increase for chemokine (C-X-C) ligand 1 growth-regulated protein alpha CXCL1 (GRO). Further analyses demonstrated that patients with UTI had significantly higher levels of GRO and interleukin-8 (IL-8) in comparison to control, nonulcerative IC, OAB wet, and bladder cancer. Both are chemokines that stimulate chemotaxis resulting in the rapid accumulation of immune cells such as neutrophils. IL-6 levels overall were at the lower limit of assay range but were significantly increased in urine of UTI patients versus IC patients. MCP-1 (CCL2) had the least separation among the control group and the various bladder diseases. Conclusion: Urinary concentrations of GRO were higher in disease state compared to control. Specifically, levels of GRO and IL-8 were higher in urine samples from patients with UTI compared to controls and other bladder conditions. Comparing and contrasting urinary cytokines may help improve our understanding of these important bladder diseases with great unmet needs.
比较间质性膀胱炎、膀胱过动症、尿路感染和膀胱癌患者尿炎性细胞因子的浓度
目的:我们试图确定尿细胞因子浓度谱在不同的膀胱条件下是否不同。材料和方法:三个临床站点的参与者完成了人口统计调查,并在含有室温尿液防腐剂的收集杯中提供了尿液样本。参与者根据医生记录的诊断分为以下类别:无症状控制,非溃疡性间质性膀胱炎(IC),膀胱过度活动并失禁(OAB湿),尿路感染(UTI)和膀胱癌。尿细胞因子检测采用Luminex多重检测法。结果:在3个临床点采集尿样277份。尿促炎细胞因子在膀胱疾病中与对照组相比有增加的趋势,其中趋化因子(C-X-C)配体1生长调节蛋白α CXCL1 (GRO)显著增加。进一步的分析表明,与对照组、非溃疡性IC、OAB湿和膀胱癌相比,UTI患者的GRO和白细胞介素-8 (IL-8)水平明显更高。两者都是刺激趋化性的趋化因子,导致免疫细胞如中性粒细胞的快速积累。总体而言,IL-6水平处于检测范围的下限,但尿路感染患者的尿液中IL-6水平明显高于IC患者。MCP-1 (CCL2)在对照组和各种膀胱疾病中分离最少。结论:疾病状态下尿GRO浓度高于对照组。具体来说,与对照组和其他膀胱疾病相比,尿路感染患者尿液样本中的GRO和IL-8水平更高。比较和对比尿细胞因子可能有助于提高我们对这些重要的膀胱疾病的认识。
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来源期刊
Urological Science
Urological Science UROLOGY & NEPHROLOGY-
CiteScore
1.20
自引率
0.00%
发文量
26
审稿时长
6 weeks
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