The viability of cell that encapsulated in calcium alginate hydrogel beads

IF 1.9 4区材料科学 Q3 Materials Science

Science and Engineering of Composite Materials Pub Date : 2022-01-01 DOI:10.1515/secm-2022-0156

Fang-fang Li, W. Tang, Q. Xie

引用次数: 0

Abstract

Abstract Objective To prove that calcium alginate beads can be used as scaffolds during in vitro culture. Methods Mouse preosteoblastic cells (MC3T3-E1) were encapsulated in calcium alginate hydrogel beads. The Cell Counting Kit-8 (CCK-8) assay was used to assess cell viability at 2, 5, 8, 11, 14, and 21 days. Calcein-AM and propidium iodide (PI) were employed for live/dead staining. Results MC3T3-E1 cells were alive on day 21 and had the highest viability on day 14. Conclusion MC3T3-E1 cells could be encapsulated in calcium alginate hydrogel beads and cultured. Calcium alginate hydrogel beads can be used as scaffolds for three-dimensional in vitro culture.

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海藻酸钙水凝胶珠包裹细胞的活力

【摘要】目的证明海藻酸钙微球可作为体外培养的支架材料。方法将小鼠成骨前细胞(MC3T3-E1)包埋在海藻酸钙水凝胶珠中。采用细胞计数试剂盒-8 (CCK-8)法评估细胞在2、5、8、11、14和21天的活力。钙黄素- am和碘化丙啶(PI)进行活/死染色。结果MC3T3-E1细胞在第21天存活，第14天细胞活力最高。结论MC3T3-E1细胞可包被在海藻酸钙水凝胶珠中培养。海藻酸钙水凝胶珠可以作为体外三维培养的支架。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Science and Engineering of Composite Materials 工程技术-材料科学：复合

CiteScore

3.10

自引率

5.30%

发文量

审稿时长

4 months

期刊介绍： Science and Engineering of Composite Materials is a quarterly publication which provides a forum for discussion of all aspects related to the structure and performance under simulated and actual service conditions of composites. The publication covers a variety of subjects, such as macro and micro and nano structure of materials, their mechanics and nanomechanics, the interphase, physical and chemical aging, fatigue, environmental interactions, and process modeling. The interdisciplinary character of the subject as well as the possible development and use of composites for novel and specific applications receives special attention.