{"title":"Classical morphology and DNA barcoding based identification of freshwater ectoparasite, Argulus foliaceus in rohu Labeo rohita","authors":"Snatashree Mohanty, Mausumee Mohanty, K. Sarma, Tarkeshwar Kumar, Amitav Dey, Paramananda Das, Sanjeeb Kumar Sahoo","doi":"10.21077/ijf.2023.70.1.132415-16","DOIUrl":null,"url":null,"abstract":"DNA barcoding coupled with classical morphological description have become promising approach for the species levelidentification. In the present study, 278 numbers of rohu Labeo rohita were screened, for Argulus infestation, amongwhich 167 fish (60.07%) were found infested with Argulus. Morphological attributes of the parasites were studied by lightmicroscope (LM) and Scanning Electron Microscope (SEM) which showed dorsoventrally flattened body comprising ofhead, thorax, abdomen, a pair of large compound eyes, suctorial organs with sclerotised support structures and two openingsof spermatheca at posterior end. Posterior incision of abdomen do not reach the mid-line and the cephalo-thoracic carapacehave not extended beyond the beginning of abdomen. Further validation was done using molecular tools for accurateparasite identification. The pair-wise genetic distance value, using Kimura-2 parameter showed a species level variationof 0.001 (1%) with that of A. foliaceus, while 0.083 and 0.052 (i.e. more than 2%) with that of A. indicus and A. japonicusrespectively. Phylogenetic tree generated using Neighbour-Joining (NJ) and Maximum-Likelihood (ML) methods, withKimura-2 parameter were also in agreement with pairwise distance values. The mitochondrial cytochrome c oxidase subunit1 (COI) sequences of A. foliaceus formed one cluster with the present studied samples, whereas sequences of A. japonicusformed a sister group. Integrating morphological and rapid DNA barcoding tools, the species was delineated as A. foliaceus.\nKeywords: Argulus foliaceus, COI gene, DNA barcoding, Labeo rohita, Morphology","PeriodicalId":50372,"journal":{"name":"Indian Journal of Fisheries","volume":" ","pages":""},"PeriodicalIF":0.4000,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indian Journal of Fisheries","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.21077/ijf.2023.70.1.132415-16","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"FISHERIES","Score":null,"Total":0}
引用次数: 0
Abstract
DNA barcoding coupled with classical morphological description have become promising approach for the species levelidentification. In the present study, 278 numbers of rohu Labeo rohita were screened, for Argulus infestation, amongwhich 167 fish (60.07%) were found infested with Argulus. Morphological attributes of the parasites were studied by lightmicroscope (LM) and Scanning Electron Microscope (SEM) which showed dorsoventrally flattened body comprising ofhead, thorax, abdomen, a pair of large compound eyes, suctorial organs with sclerotised support structures and two openingsof spermatheca at posterior end. Posterior incision of abdomen do not reach the mid-line and the cephalo-thoracic carapacehave not extended beyond the beginning of abdomen. Further validation was done using molecular tools for accurateparasite identification. The pair-wise genetic distance value, using Kimura-2 parameter showed a species level variationof 0.001 (1%) with that of A. foliaceus, while 0.083 and 0.052 (i.e. more than 2%) with that of A. indicus and A. japonicusrespectively. Phylogenetic tree generated using Neighbour-Joining (NJ) and Maximum-Likelihood (ML) methods, withKimura-2 parameter were also in agreement with pairwise distance values. The mitochondrial cytochrome c oxidase subunit1 (COI) sequences of A. foliaceus formed one cluster with the present studied samples, whereas sequences of A. japonicusformed a sister group. Integrating morphological and rapid DNA barcoding tools, the species was delineated as A. foliaceus.
Keywords: Argulus foliaceus, COI gene, DNA barcoding, Labeo rohita, Morphology
期刊介绍:
Indian Journal of Fisheries is published quarterly by the Indian Council of Agricultural Research (ICAR), New Delhi. Original contributions in the field of Fish and fisheries science are considered for publication in the Journal. The material submitted must be unpublished and not under consideration for publication elsewhere.
Papers based on research which kills or damages any species, regarded as thratened/ endangered by IUCN crieteria or is as such listed in the Red Data Book appropriate to the geographic area concerned, will not be accepted by the Journal, unless the work has clear conservation objectives.