Extracts of Tridax procumbens linn leaves causes wound healing in diabetic and Non-diabetic laboratory animals

Abstract

Background

Tridax procumbens(L.) leaves are traditionally used in wound healing by tribal people. The matured leaves are crushed to make a paste and applied on the surface of the wound.

Purpose

The aim of the present investigation was to evaluate the wound healing activity of ethanolic extract of Tridax procumbens (L.) (EETP) in streptozotocin induced diabetic and non- diabetic laboratory animals. There is no scientific literature, claiming the use of leaves of Tridax procumbens (L.) in diabetic wounds.

Study Design

The simple ointment base (2.5 % and 5% w/w) of EETP was formulated to evaluate the wound healing potential on diabetic and non-diabetic rats, using incision, excision and burn wound model.

Methods

In incision wound model, epithelization period, wound index, % contraction area, hydroxyproline content, DNA estimation, total protein and histopathological evaluations were done. In excision wound model, tensile strength was measured. In burn model, contraction rate, wound contraction (%) and epithelization period were evaluated.

Results

Secondary metabolite as tannin, flavonoids were present. In the excision model, animals treated with 2.5 % and 5% w/w EETP showed significant result in wound contraction, epithelization period and wound index. Hydroxyproline, total protein and DNA concentration in healing tissue were more in comparison to control and as well as standard in diabetic and non- diabetic. The parameters evaluated showed significant response at 2.5 % and 5% w/w EETP.

Conclusion

Flavonoids and tannin were accountable for wound healing. The findings confirmed the ethanomedicinal claim of Tridax procumbens(L.) in wound healing in diabetic and nondiabetic conditions.