High-efficiency oxygen evolution by photosystem II oxygen-evolving complex containing 3Mn per reaction center

IF 2.7 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

JBIC Journal of Biological Inorganic Chemistry Pub Date : 2023-04-12 DOI:10.1007/s00775-023-01987-2

Boris К. Semin, Lira N. Davletshina

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Abstract

Ca-depleted photosystem II membranes obtained by treatment with acidic buffer do not contain Ca²⁺ in the Mn₄CaO₅ cluster but contain all extrinsic proteins protecting this cluster (PSII(-Ca/low pH)). However, unlike native photosystem II, Mn cluster in PSII(-Ca/low pH) samples is available for small-sized reductants. Using this property, we investigated the substitution possibility of Mn cation(s) with Fe cation(s) to obtain a chimeric cluster in PSII(-Ca/low pH) samples containing extrinsic proteins. We found that Fe(II) cation replaces Mn cation at pH 6.5, however, PSII(-Ca/low pH) membranes with the 3Mn1Fe chimeric cluster in the oxygen-evolving complex evolve O₂ with high intensity in the presence of exogenous Ca²⁺. The O₂ evolution rate is about 80% of the same rate in PSII(-Ca/low pH) membranes.

Graphical abstract

Abstract Image

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光系统高效析氧ⅱ析氧配合物，每个反应中心含3Mn

通过酸性缓冲液处理获得的光系统II膜在Mn4CaO5簇中不含Ca2+，但含有保护该簇的所有外源蛋白(PSII(-Ca/低pH值))。然而，与原生光系统II不同，PSII(-Ca/低pH)样品中的Mn簇可用于小尺寸还原剂。利用这一性质，我们研究了在含有外源蛋白的PSII(-Ca/低pH)样品中，Mn阳离子与Fe阳离子取代获得嵌合簇的可能性。我们发现Fe(II)阳离子在pH为6.5时取代Mn阳离子，然而，PSII(-Ca/低pH)膜在外源Ca2+存在下，具有3Mn1Fe嵌合簇的氧进化复合物中以高强度进化O2。在PSII(-Ca/低pH)膜中，O2的析出率约为相同速率的80%。图形抽象

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来源期刊

JBIC Journal of Biological Inorganic Chemistry 化学-生化与分子生物学

CiteScore

5.90

自引率

3.30%

发文量

审稿时长

3 months

期刊介绍： Biological inorganic chemistry is a growing field of science that embraces the principles of biology and inorganic chemistry and impacts other fields ranging from medicine to the environment. JBIC (Journal of Biological Inorganic Chemistry) seeks to promote this field internationally. The Journal is primarily concerned with advances in understanding the role of metal ions within a biological matrix—be it a protein, DNA/RNA, or a cell, as well as appropriate model studies. Manuscripts describing high-quality original research on the above topics in English are invited for submission to this Journal. The Journal publishes original articles, minireviews, and commentaries on debated issues.