Small colony variants of Burkholderia pseudomallei: alteration of the virulence factors

IF 2.8 3区综合性期刊 Q2 MULTIDISCIPLINARY SCIENCES

Journal of Taibah University for Science Pub Date : 2023-08-14 DOI:10.1080/16583655.2023.2244657

Vanitha Mariappan, M. Barathan, A. K. Zulpa, J. Vadivelu, K. M. Vellasamy

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引用次数: 0

Abstract

Burkholderia pseudomallei produce small colony variants (SCVs) that can persist in harsh conditions. This study aimed to compare SCV with B. pseudomallei wild-type (WT) in the ability to adhere, invade, survive and produce biofilm. Additionally, proteins responsible for pathogenicity were determined through two-dimensional gel electrophoresis (2-DE) and liquid-chromatography mass-spectrometry (LC-MS). Whole bacterial proteins analysed using 2-DE demonstrated 252 and 323 protein spots in WT and SCV, respectively, with 37 distinctive proteins identified in the SCV. Several isoform proteins (dnaK, groEL1, gapA and tuf), upregulated in SCV, may function as moonlighting proteins. Quantitative proteomic analysis using LC-MS revealed 57 and 99 distinctive proteins in the WT and SCV, respectively. Many distinct proteins communicated by B. pseudomallei SCV were involved in the metabolic pathways and may play a role in the alteration of virulence factors. Thus, the alteration of proteins in SCVs may be an approach of B. pseudomallei to enhance pathogenesis.

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来源期刊

Journal of Taibah University for Science MULTIDISCIPLINARY SCIENCES-

CiteScore

6.60

自引率

6.10%

发文量

102

审稿时长

19 weeks

期刊介绍： Journal of Taibah University for Science (JTUSCI) is an international scientific journal for the basic sciences. This journal is produced and published by Taibah University, Madinah, Kingdom of Saudi Arabia. The scope of the journal is to publish peer reviewed research papers, short communications, reviews and comments as well as the scientific conference proceedings in a special issue. The emphasis is on biology, geology, chemistry, environmental control, mathematics and statistics, nanotechnology, physics, and related fields of study. The JTUSCI now quarterly publishes four issues (Jan, Apr, Jul and Oct) per year. Submission to the Journal is based on the understanding that the article has not been previously published in any other form and is not considered for publication elsewhere.