Haplotype Diversity and Molecular Phylogeny of Wild Crambe L. (Brassicaceae) Taxa of Turkey

IF 1.5 4区生物学 Q3 PLANT SCIENCES

Turkish Journal of Botany Pub Date : 2023-01-01 DOI:10.55730/1300-008x.2766

Burcu Hacıoğlu, Kurtulus Özgisi

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引用次数: 0

Abstract

: Revealing genetic diversity is both essential for plant systematics and also provides important information for agricultural sciences. Crambe hispanica var. abyssinica is an oil seed crop. Wild plants related to crops (Crop Wild Relatives) are important resources for the genetic improvement of cultivated species. In order to reveal the genetic diversity of wild Crambe taxa of Turkey, we used ITS and trn L-F markers to create the haplotype networks and phylogeny reconstruction. Thirty-nine accessions belonging to 8 Crambe taxa were used as the material of our study: Crambe orientalis var . orientalis with 18 accessions, Crambe orientalis var . sulphurea with 3 accessions, Crambe orientalis var . dasycarpa with 1 accession, Crambe alutacea with 1 accession, Crambe grandiflora with 1 accession, Crambe tataria var . tataria with 8 accessions, Crambe tataria var. aspera with 2 accessions, and Crambe maritima with 5 accessions. The phylogeny inference of ITS and trn L-F data revealed two major lineages: one consisted of Crambe maritima and Crambe tataria accessions, while the other consisted of Crambe orientalis subsp . orientalis , Crambe orientalis subsp . sulphurea , Crambe orientalis var . dasycarpa , Crambe grandiflora , and Crambe alutacea. In the haplotype networks based trn L-F region, all Crambe maritima accessions and most of the Crambe. orientalis accessions shared one haplotype. However, Crambe maritima , Crambe orientalis , and Crambe tataria shared no common haplotype in the networks based on ITS region. In both networks, Crambe alutacea shared one haplotype with some Crambe orientalis . Crambe maritima and Crambe orientalis shared the H1 haplotype . Other haplotypes differed from the most common haplotype (H1) by one or two base pairs. Crambe orientalis is the species with the highest haplotype diversity and IT6 haplotype has the highest seed oil content among CWR of Crambe in Turkey.

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土耳其野生芸苔科Crambe L.分类群的单倍型多样性和分子系统发育

揭示遗传多样性是植物系统学研究的重要内容，也是农业科学研究的重要信息来源。海芋是一种油料种子作物。与作物有关的野生植物(作物近缘种)是栽培种遗传改良的重要资源。为了揭示土耳其野生克兰贝类群的遗传多样性，我们利用ITS和trn L-F标记建立了单倍型网络并进行了系统发育重建。本研究以8个克拉姆分类群的39份材料为材料:克拉姆(Crambe orientalis var);东方学有18种，东方学有18种。硫脲与3个加入，克兰东方品种。山楂1株，鹤耳草1株，桔梗1株，鹤耳草1株。鞑靼菜有8种，鞑靼菜有2种，海苔菜有5种。ITS和trn L-F数据的系统发育推断揭示了两个主要谱系:一个是由克拉姆和鞑靼系组成的，另一个是由克拉姆东方亚系组成的。东洋蓟，东洋蓟亚种。硫脲，东方克兰变种。山菖蒲、大花菖蒲和花菖蒲。在以trn - f区为基础的单倍型网络中，所有的克兰伯海洋物种和大部分克兰伯海洋物种。东方人的遗传资料共有一个单倍型。然而，在基于ITS区域的网络中，克拉姆、东方克拉姆和鞑靼克拉姆没有共同的单倍型。在这两个网络中，蓝刺鹤与一些东方鹤共有一个单倍型。海蛤和东方蛤具有相同的H1单倍型。其他单倍型与最常见的单倍型(H1)有一个或两个碱基对的差异。在土耳其克兰贝CWR中，克兰贝单倍型多样性最高，IT6单倍型种子含油量最高。

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来源期刊

Turkish Journal of Botany PLANT SCIENCES-

CiteScore

2.90

自引率

5.60%

发文量

审稿时长

6-12 weeks

期刊介绍： The Turkish Journal of Botany is published electronically 6 times a year by the Scientific and Technological Research Council of Turkey (TÜBİTAK) and accepts manuscripts (in English) covering all areas of plant biology (including genetics, evolution, systematics, structure, function, development, diversity, conservation biology, biogeography, paleobotany, ontogeny, functional morphology, ecology, reproductive biology, and pollination biology), all levels of organisation (molecular to ecosystem), and all plant groups and allied organisms (algae, fungi, and lichens). Authors are required to frame their research questions and discuss their results in terms of major questions in plant biology. In general, papers that are too narrowly focused, purely descriptive, or broad surveys, or that contain only preliminary data or natural history, will not be considered (*). The following types of article will be considered: 1. Research articles: Original research in various fields of botany will be evaluated as research articles. 2. Research notes: These include articles such as preliminary notes on a study or manuscripts on the morphological, anatomical, cytological, physiological, biochemical, and other properties of plant, algae, lichen and fungi species. 3. Reviews: Reviews of recent developments, improvements, discoveries, and ideas in various fields of botany. 4. Letters to the editor: These include opinions, comments relating to the publishing policy of the Turkish Journal of Botany, news, and suggestions. Letters should not exceed one journal page. (*) 1. Raw floristic lists (of algae, lichens, fungi, or plants), species descriptions, chorological studies, and plant sociology studies without any additional independent approaches. 2. Comparative morphology and anatomy studies (that do not cover a family, tribe, subtribe, genus, subgenus, section, subsection, or species complexes with taxonomical problems) without one or more independent additional approaches such as phylogenetical, micromorphological, chromosomal and anatomical analyses. 3. Revisions of family, tribe, genus, subgenus, section, subsection, or species complexes without any original outputs such as taxonomical status changes, IUCN categories, and phenological and ecological analyses. 4. New taxa of all plants without any additional independent approaches such as phylogenetical, ecological, chromosomal, chorological and correlational analyses in addition to a detailed macro- and micro-morphological descriptions with quality field and microscopic illustrations of taxonomically important structures and identification key in the taxonomic group. New records of all plants without any additional independent approaches such as phylogenetical, ecological, chromosomal, chorological and correlational analyses in addition to a detailed macro- and micro-morphological descriptions with quality field and microscopic illustrations of taxonomically important structures and identification key in the taxonomic group may be accepted for peer review if they contain 3 or more new records or taxonomical status update, such as lectotypification, new combinations, transfers, revivals and synonyms. 5. New taxa of algae, lichens, and fungi without any additional independent approaches such as phylogenetical, ecological, chromosomal, chorological and correlational analyses in addition to a detailed macro- and micro-morphological descriptions with quality field and microscopic illustrations of taxonomically important structures and identification key in the taxonomic group. New records of algae, lichens, and fungi without any additional independent approaches such as phylogenetical, ecological, chromosomal, chorological and correlational analyses in addition to a detailed macro- and micro-morphological descriptions with quality field and microscopic illustrations of taxonomically important structures and identification key in the taxonomic group may be accepted for peer review if they contain 5 or more new records or taxonomical status update, such as lectotypification, new combinations, transfers, revivals and synonyms.