Statistical optimization of enzymatic saccharification of sodium hydroxide pretreated parthenium hysterophorus biomass using response surface methodology

IF 1.7 4区农林科学 Q2 MATERIALS SCIENCE, PAPER & WOOD

Journal of Wood Chemistry and Technology Pub Date : 2022-11-14 DOI:10.1080/02773813.2022.2145312

Naveen Kumar, Mahak Mittal, A. Yadav, D. Saini, N. Aggarwal

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引用次数: 4

Abstract

Abstract The biomass of the lignocellulosic weed P. hysterophorus is abundant and wasteful in India. This study aimed to investigate the lignocellulosic weed P. hysterophorus for reducing sugar production. Therefore, several pretreatment methods, physical (Steam and Microwave), and chemical (alkalis and acids) were investigated to determine the statistically best conditions for enzymatic hydrolysis. The results revealed that alkali pretreatment (sodium hydroxide, 1.0% w/v) had the greatest impact on the production of reducing sugar (110.36 ± 0.05 mg/g substrate) from P. hysterophorus. The decrease in the lignin content and increase in carbohydrates from pretreated biomass was further validated using an ultrastructural technique (Fourier-transform infrared spectroscopy and X-ray diffraction). The quantity of reducing sugars released was optimized using a response surface methodology, and the experimental results with optimized process variables gave 385.44 mg/g substrate reducing sugar. The investigations showed that P. hysterophorus can be utilized as a promising and low-cost biomass source for commercial ethanol production. Graphical abstract

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响应面法统计优化氢氧化钠预处理的白头翁生物量的酶糖化

在印度，木质纤维素杂草P. hysterophorus的生物量丰富而浪费。本研究旨在研究木质纤维素杂草子宫草(P. hysterophorus)的还原性。因此，研究了几种预处理方法，物理预处理(蒸汽和微波)和化学预处理(碱和酸)，以确定统计学上最佳的酶解条件。结果表明，碱预处理(氢氧化钠，1.0% w/v)对宫参产还原糖(110.36±0.05 mg/g底物)的影响最大。利用超微结构技术(傅里叶变换红外光谱和x射线衍射)进一步验证了预处理生物质木质素含量的降低和碳水化合物含量的增加。采用响应面法对还原糖的释放量进行优化，优化后的实验结果为385.44 mg/g底物还原糖。研究表明，子宫草可作为一种有前景的低成本生物质资源用于商业乙醇生产。图形抽象

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来源期刊

Journal of Wood Chemistry and Technology 工程技术-材料科学：纸与木材

CiteScore

3.70

自引率

20.00%

发文量

审稿时长

3 months

期刊介绍： The Journal of Wood Chemistry and Technology (JWCT) is focused on the rapid publication of research advances in the chemistry of bio-based materials and products, including all aspects of wood-based polymers, chemicals, materials, and technology. JWCT provides an international forum for researchers and manufacturers working in wood-based biopolymers and chemicals, synthesis and characterization, as well as the chemistry of biomass conversion and utilization. JWCT primarily publishes original research papers and communications, and occasionally invited review articles and special issues. Special issues must summarize and analyze state-of-the-art developments within the field of biomass chemistry, or be in tribute to the career of a distinguished researcher. If you wish to suggest a special issue for the Journal, please email the Editor-in-Chief a detailed proposal that includes the topic, a list of potential contributors, and a time-line.