Association Between the -1031 (T/C) Polymorphism of TNF-α Gene and Biochemical Factors in Women With Polycystic Ovary Syndrome

M. Sahmani, T. D. Ghaleh, Maryam Yargholi, Farshad Foroghi, A. Javadi, Khadijeh Taherkhani
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Abstract

Background: Tumor Necrosis Factor Alpha (TNF-α) gene, as an inflammatory factor, plays an important role in reproductive physiology, especially in women with Polycystic Ovary Syndrome (PCOS). Objective: This study aims to investigate the relationship between the -1031 T/C polymorphism of TNF-α gene and biochemical factors in women with PCOS. Methods: In this case-control study, participants were 106 women with PCOS and 114 healthy women referred to Kosar Hospital in Qazvin, Iran. The TNF-α gene’s polymorphism was determined using Polymerase Chain Reaction (PCR) technique and PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) method. Biochemical factors of serum levels were also measured in two groups. Logistic regression analysis examined the relationship between the frequency of alleles in different states and the risk of PCOS. Findings: There were statistically significant difference in the mean levels of total cholesterol, triglycerides, testosterone, two-hour blood glucose and body mass index between the groups, whose values were higher in women with PCOS compared to healthy women (P<0.001). In women with PCOS, the mean serum levels of triglyceride and high-density lipoprotein were significantly different between the three TT, CC, TC genotypes of TNF-α gene polymorphism (P<0.05). The results of regression analysis showed that the TT genotype had significant association with the risk of PCOS (OR=2.43, P=0.006, 95%CI: 1.28-2.62). Conclusion: It seems that there is a relationship between the -1301 (T/C) polymorphism of TNF-α gene and the risk of PCOS in women.
多囊卵巢综合征患者TNF-α基因-1031(T/C)多态性与生化因素的相关性研究
背景:肿瘤坏死因子α (Tumor Necrosis Factor α, TNF-α)基因作为一种炎症因子,在生殖生理,尤其是多囊卵巢综合征(PCOS)女性中起着重要作用。目的:探讨多囊卵巢综合征(PCOS)患者TNF-α基因-1031 T/C多态性与生化因素的关系。方法:在这项病例对照研究中,参与者是106名多囊卵巢综合征妇女和114名转诊到伊朗加兹温Kosar医院的健康妇女。采用聚合酶链反应(PCR)技术和PCR-限制性片段长度多态性(PCR- rflp)方法检测TNF-α基因多态性。同时测定两组患者血清生化指标。Logistic回归分析不同状态等位基因频率与PCOS发病风险的关系。结果:两组间总胆固醇、甘油三酯、睾酮、两小时血糖和体重指数的平均水平有统计学差异,多囊卵巢综合征女性的这些数值高于健康女性(P<0.001)。PCOS患者血清中甘油三酯和高密度脂蛋白水平在TNF-α基因多态性TT、CC、TC三种基因型之间差异均有统计学意义(P<0.05)。回归分析结果显示TT基因型与PCOS发病风险有显著相关性(OR=2.43, P=0.006, 95%CI: 1.28 ~ 2.62)。结论:TNF-α基因-1301 (T/C)多态性可能与女性PCOS发病风险有关。
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