Variation of Proconvertase 1 and Resistin Gene as Risk Factor for Type II Diabetes Mellitus in Obesity Papua Population

Malaysian Journal of Medicine and Health Sciences Pub Date : 2023-05-15 DOI:10.47836/mjmhs.19.3.37

P. Hastuti, A. Sadewa, Jems K. R. Maay

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Abstract

Introduction: Risk factors for type 2 diabetes mellitus (T2DM) include obesity and some genetic factors. Obesity involves mild chronic inflammation that predisposes cells to insulin resistance. Two genes that influence obesity and insulin resistance are Proconvertase-1 (PC-1) and resistin (RETN). PC-1 affects the activation of hormones that regulate satiety and hunger. Resistin is one of the inflammatory factors that influence the occurrence of insulin resistance. This study aimed to determine the influence of polymorphism in the PC-1 gene rs1044498 (C>A) and resistin gene RETN + 299 G>A rs3745367 on the risk of diabetes in obese Papua population. Methods: This study involved 58 obese people with T2DM and 58 obese people without DM. We examined the characteristics of blood pressure, lipid profile and insulin resistance by HOMA-IR. The genes examined were PC-1 rs1044498 (C>A) and RETN+ 299 G>A rs3745367 by the PCR-RFLP method. The relationship of gene variations with biochemical parameters was determined with analysis of variance. The results were considered significantly different if P < 0.05. Results: In this study, parameters of diastolic blood pressure, triglycerides and insulin resistance were higher while high density lipoprotein (HDL) levels were lower and significantly different in the obese with T2DM group compared to the obese only group. The carrier of the A allele in the PC-1 gene rs1044498 was higher in the obese group than the obese with T2DM but not significantly different in biochemical parameters. Carrier of the AA genotype in the RETN gene + 299 G>A rs3745367 had higher triglycerides and HOMA-IR and lower HDL levels significantly different (P<0.05) than other genotypes in the obesity with T2DM group. Conclusion: PC-1 rs1044498 gene was a risk factor for obesity but not for T2DM, while RETN gene rs3745367 was a risk factor for dyslipidemia and diabetes in obese people in the Papua population.

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巴布亚肥胖人群2型糖尿病的危险因素转化酶1和抵抗素基因变异

2型糖尿病(T2DM)的危险因素包括肥胖和一些遗传因素。肥胖涉及轻微的慢性炎症，使细胞容易产生胰岛素抵抗。影响肥胖和胰岛素抵抗的两个基因是转化酶原-1 (PC-1)和抵抗素(RETN)。PC-1影响调节饱腹感和饥饿感的激素的激活。抵抗素是影响胰岛素抵抗发生的炎症因子之一。本研究旨在确定PC-1基因rs1044498 (C>A)和抵抗素基因RETN + 299 G>A rs3745367多态性对肥胖巴布亚人群糖尿病风险的影响。方法:本研究纳入58例肥胖者合并2型糖尿病和58例肥胖者非糖尿病，通过HOMA-IR检测血压、血脂和胰岛素抵抗的特征。PCR-RFLP方法检测的基因为PC-1 rs1044498 (C b> A)和RETN+ 299 G>A rs3745367。通过方差分析确定基因变异与生化参数的关系。以P < 0.05为差异有统计学意义。结果:在本研究中，肥胖合并T2DM组的舒张压、甘油三酯、胰岛素抵抗等指标均高于单纯肥胖组，高密度脂蛋白(HDL)水平低于单纯肥胖组，且差异有统计学意义。肥胖组PC-1基因rs1044498 A等位基因携带者高于肥胖合并T2DM组，但生化指标差异无统计学意义。RETN基因+ 299 G>A rs3745367 AA基因型携带者与其他基因型相比，肥胖合并T2DM组甘油三酯、HOMA-IR升高，HDL降低，差异有统计学意义(P<0.05)。结论:PC-1 rs1044498基因是肥胖的危险因素，但不是T2DM的危险因素，而RETN基因rs3745367是巴布亚人群肥胖人群血脂异常和糖尿病的危险因素。

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来源期刊

Malaysian Journal of Medicine and Health Sciences Medicine-Medicine (all)

CiteScore

0.50

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0.00%

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期刊介绍： The Malaysian Journal of Medicine and Health Sciences (MJMHS) is published by the Faculty of Medicine and Health Sciences, Universiti Putra Malaysia. The main aim of the MJMHS is to be a premier journal on all aspects of medicine and health sciences in Malaysia and internationally. The focus of the MJMHS will be on results of original scientific research and development, emerging issues and policy analyses pertaining to medical, biomedical and clinical sciences.