Apoptotic-based topotecan-loaded superparamagnetic drug delivery system: an in vitro study in MCF7

IF 1.3 4区工程技术 Q4 ENGINEERING, BIOMEDICAL

Bioinspired Biomimetic and Nanobiomaterials Pub Date : 2022-11-07 DOI:10.1680/jbibn.21.00053

Niyousha Yazdanmehr, M. Tajabadi, R. Bigdeli, Hanif Goran Orimi, V. Asgary

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引用次数: 0

Abstract

Biological barriers could be overcome using nano-biotechnology, which promotes the development of nanomaterial-based delivery systems. The primary objective of the present investigation focuses on superparamagnetic iron oxide nanoparticle (SPION) production for the delivery of topotecan to human breast cancer cells (MCF-7). The XRD results confirm the formation of pure SPION. The FTIR spectra indicate the functional groups related to aminopropyl trimethoxy silane (APTS) as a coating agent and topotecan. Topotecan-loaded magnetite nanoparticles with an IC50 of approximately 156 µg/mL exhibited dose-dependent cytotoxicity. The PCR method also proved that, in the mentioned cell line, topotecan-loaded SPION could increase the Bax/Bcl2 ratio and P53 gene expression. Annexin V/PI detection assay was done in order to detect the induction of apoptosis. According to the results, the nanoparticles inhibitively influence the survival of the MCF-7 breast cancer cells via boosting apoptosis, which helps to slow the growth of tumor cells.

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基于凋亡的拓扑替康超顺磁性给药系统：MCF7的体外研究

使用纳米生物技术可以克服生物障碍，这促进了基于纳米材料的递送系统的发展。本研究的主要目的集中于超顺磁性氧化铁纳米颗粒（SPION）的生产，用于将拓扑替康递送至人类乳腺癌症细胞（MCF-7）。XRD结果证实了纯SPION的形成。FTIR光谱显示了与氨基丙基三甲氧基硅烷（APTS）作为涂层剂和拓扑替康相关的官能团。拓扑替康负载的磁铁矿纳米颗粒，IC50约为156 µg/mL表现出剂量依赖性细胞毒性。PCR方法还证明，在上述细胞系中，拓扑替康负载的SPION可以提高Bax/Bcl2比率和P53基因的表达。采用膜联蛋白V/PI检测法检测细胞凋亡的诱导作用。根据研究结果，纳米颗粒通过促进细胞凋亡来抑制MCF-7乳腺癌症细胞的存活，这有助于减缓肿瘤细胞的生长。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioinspired Biomimetic and Nanobiomaterials ENGINEERING, BIOMEDICAL-MATERIALS SCIENCE, BIOMATERIALS

CiteScore

2.20

自引率

0.00%

发文量

期刊介绍： Bioinspired, biomimetic and nanobiomaterials are emerging as the most promising area of research within the area of biological materials science and engineering. The technological significance of this area is immense for applications as diverse as tissue engineering and drug delivery biosystems to biomimicked sensors and optical devices. Bioinspired, Biomimetic and Nanobiomaterials provides a unique scholarly forum for discussion and reporting of structure sensitive functional properties of nature inspired materials.