IN VITRO PROPAGATION TECHNOLOGY FOR THE BLACK CHOKEBERRY ARONIA MELANOCARPA (Michx.) ELLIOT

Q4 Agricultural and Biological Sciences

Fruit Growing Research Pub Date : 2022-12-22 DOI:10.33045/fgr.v38.2022.29

V. Isac, C. Plopa

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Abstract

The fruits of Aronia melanocarpa (Michx.) Elliot are recognized for their many benefits on the human body due to their medicinal and nutritional qualities. In order to ensure the need for propagating material recently requested by the market, an attempt was made to make the multiplication more efficient. Classical methods of multiplication have given poor results, which has led to the approach of multiplication by in vitro cultures. The varieties of interest were Nero and Melrom. The research carried out so far has established effective culture media for the initiation, multiplication and rooting phases as follows: MS mineral salts and LS vitamins with a hormonal balance consisting of GAɜ, BAP and IBA for the initiation and multiplication stages. The phytohormones GAɜ and IBA were used for rooting. The regeneration of the explants in the initiation stage reached an average of 86%. The multiplication rate was on average at Nero of 6 shoots / explant and at Melrom 12.6 shoots / explant. The rooting on the established in vitro culture media was 97% for Nero and 80.19% for Melrom. The acclimatization percentages were 92.15% for Nero and 90.41% for Melrom. In vitro propagation of Aronia melanocarpa (Michx.) Elliot has proven to be the method that gives the best results for the multiplication of this species.

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黑草莓（密歇根州）ELLIOT的离体繁殖技术

黑桫椤的果实(micx .)艾略特因其药用和营养价值而被公认对人体有许多好处。为了保证市场最近对宣传材料的需求，我们尝试使乘法更有效率。经典的增殖方法结果不佳，这导致了体外培养增殖的方法。不同的兴趣是尼禄和梅洛姆。目前已确定的萌发、增殖和生根阶段的有效培养基为:MS无机盐和LS维生素，激素平衡为GA / BAP / IBA。用植物激素GA / a和IBA进行生根。萌发期外植体的再生率平均达到86%。尼禄的平均增殖率为6个芽/外植体，梅尔罗姆的平均增殖率为12.6个芽/外植体。在已建立的离体培养基上，Nero和Melrom的生根率分别为97%和80.19%。Nero和Melrom的驯化率分别为92.15%和90.41%。黑桫椤离体繁殖的研究艾略特的方法已经被证明是这个物种繁殖的最佳方法。

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来源期刊

Fruit Growing Research Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

0.10

自引率

0.00%

发文量

审稿时长

8 weeks

期刊介绍： FRUIT GROWING RESEARCH is an international journal, which has been issued under the care of the Research Institute for Fruit Growing Pitesti, Romania. Fruit growing research is an annual journal aimed at disseminating significant research and original papers on genetic resources, breeding of deciduous fruit trees species; fruit sustainable technologies – environment protection; propagation, virology and tissue culture, postharvest, that can be of interest to a wide audience of plant scientists in all areas of fruit growing. An international Editorial Board advises the journal.