Genetic polymorphism of the ovine MAP3K5 gene and its association with body size traits in Hu sheep of China

IF 1.6 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Archiv Fur Tierzucht-Archives of Animal Breeding Pub Date : 2023-02-16 DOI:10.5194/aab-66-71-2023

Xiaobin Yang, Weimin Wang, Deyin Zhang, Xiaolong Li, Yukun Zhang, Yuan Zhao, Liming Zhao, Jianghui Wang, Dan Xu, Jiangbo Cheng, Wenxin Li, Bubo Zhou, Changchun Lin, X. Zeng, R. Zhai, Zongwu Ma, Jia Liu, Panpan Cui, Xiaoxue Zhang

{"title":"Genetic polymorphism of the ovine MAP3K5 gene and its association with body size traits in Hu sheep of China","authors":"Xiaobin Yang, Weimin Wang, Deyin Zhang, Xiaolong Li, Yukun Zhang, Yuan Zhao, Liming Zhao, Jianghui Wang, Dan Xu, Jiangbo Cheng, Wenxin Li, Bubo Zhou, Changchun Lin, X. Zeng, R. Zhai, Zongwu Ma, Jia Liu, Panpan Cui, Xiaoxue Zhang","doi":"10.5194/aab-66-71-2023","DOIUrl":null,"url":null,"abstract":"Abstract. As an excellent local sheep breed in China, Hu sheep have the\ncharacteristics of producing more lambs and good motherhood. The purpose of\nthis study was to identify the polymorphism of the mitogen-activated protein\nkinase 5 (MAP3K5) gene and determine whether it was associated with the body size\ntraits (body height, body length, chest circumference, and cannon\ncircumference) in Hu sheep. The polymorphism of MAP3K5 was identified by using PCR\namplification, followed by Sanger sequencing, and KASPar (Kompetitive Allele Specific PCR) technology was used for genotyping subsequently. The results of the association analysis showed\nthat MAP3K5 (g.205261 A > G) was significantly associated with body\nheight at 80, 100, 140, 160, and 180 d; body length at 80 and 160 d;\nchest circumference at 100, 140, 160, and 180 d; and cannon circumference at\n100 and 120 d, respectively. The results of qRT-PCR showed that the\nexpression level of MAP3K5 in the heart was significantly higher (P < 0.05)\nthan in the other 10 tissues. In summary, the MAP3K5 mutation loci may be used as\na candidate molecular marker for the body size traits of Hu sheep.\n","PeriodicalId":55481,"journal":{"name":"Archiv Fur Tierzucht-Archives of Animal Breeding","volume":" ","pages":""},"PeriodicalIF":1.6000,"publicationDate":"2023-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Archiv Fur Tierzucht-Archives of Animal Breeding","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.5194/aab-66-71-2023","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}

引用次数: 0

Abstract

Abstract. As an excellent local sheep breed in China, Hu sheep have the characteristics of producing more lambs and good motherhood. The purpose of this study was to identify the polymorphism of the mitogen-activated protein kinase 5 (MAP3K5) gene and determine whether it was associated with the body size traits (body height, body length, chest circumference, and cannon circumference) in Hu sheep. The polymorphism of MAP3K5 was identified by using PCR amplification, followed by Sanger sequencing, and KASPar (Kompetitive Allele Specific PCR) technology was used for genotyping subsequently. The results of the association analysis showed that MAP3K5 (g.205261 A > G) was significantly associated with body height at 80, 100, 140, 160, and 180 d; body length at 80 and 160 d; chest circumference at 100, 140, 160, and 180 d; and cannon circumference at 100 and 120 d, respectively. The results of qRT-PCR showed that the expression level of MAP3K5 in the heart was significantly higher (P < 0.05) than in the other 10 tissues. In summary, the MAP3K5 mutation loci may be used as a candidate molecular marker for the body size traits of Hu sheep.

查看原文本刊更多论文

中国湖羊MAP3K5基因遗传多态性及其与体型性状的关系

摘要湖羊是中国优良的地方绵羊品种，具有产羔多、母性好等特点。本研究的目的是鉴定有丝分裂原活化蛋白激酶5 (MAP3K5)基因多态性，并确定其是否与湖羊的体型性状(体高、体长、胸围、胸围)相关。采用PCR扩增、Sanger测序和KASPar (competitive Allele Specific PCR)技术对MAP3K5进行基因分型鉴定。关联分析结果显示，MAP3K5 (g.205261在80、100、140、160和180 d时，体重与体高显著相关;80、160天体长，100、140、160、180天胸围;和大炮周长分别在100和120 d。qRT-PCR结果显示，MAP3K5在心脏组织中的表达水平显著高于其他10个组织(P < 0.05)。综上所述，MAP3K5突变位点可作为湖羊体型性状的候选分子标记。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Archiv Fur Tierzucht-Archives of Animal Breeding 农林科学-奶制品与动物科学

CiteScore

3.20

自引率

0.00%

发文量

审稿时长

18-36 weeks

期刊介绍： Archives Animal Breeding is an open-access journal publishing original research papers, short communications, brief reports, and reviews by international researchers on scientific progress in farm-animal biology. The journal includes publications in quantitative and molecular genetics, genetic diversity, animal husbandry and welfare, physiology, and reproduction of livestock. It addresses researchers, teachers, stakeholders of academic and educational institutions, as well as industrial and governmental organizations in the field of animal production.