Antimicrobial Resistance Profile and Molecular Characterization of Staphylococcus aureus Isolated from Subclinical Mastitis of Dairy Cows in Mathura Region

Journal of dairying, foods & home sciences Pub Date : 2023-04-06 DOI:10.18805/ajdfr.dr-1953

Swati Tripathi, R. Singh, M. Srivastava, Ashutosh Kumar Singh, S. Yadav

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Abstract

Background: Subclinical mastitis is the most underrated yet economically important disease of livestock. It goes undetected by clinical examinations, making routine surveillance and monitoring necessary for its detection. Among causative agents, Staphylococcus aureus is the most crucial one. The present study determines the prevalence, antimicrobial resistance profile and molecular characterization of S. aureus from subclinical mastitis of cattle in the Mathura region. Methods: The present research was conducted during 2019-2021 and different gaushalas and dairies in and around the Mathura region were screened for mastitis by California mastitis test and somatic cell count. The samples positive were further tested by bacterial, biochemical and molecular tests along with an antimicrobial resistance profile. Result: Our research found a significant amount of S. aureus in subclinical samples with the presence of mecA gene suggesting MRSA. The public health importance of S. aureus and emerging resistance against antibiotics demands regular monitoring and effective use of antimicrobial agents against the MRSA isolates.

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马图拉地区奶牛亚临床乳腺炎金黄色葡萄球菌耐药谱及分子特征分析

背景:亚临床乳腺炎是家畜最常被低估但经济上重要的疾病。临床检查无法发现该病，因此必须进行常规监测和监测才能发现该病。其中，金黄色葡萄球菌是最重要的病原体。本研究确定了马图拉地区牛亚临床乳腺炎金黄色葡萄球菌的流行、耐药性特征和分子特征。方法:本研究于2019-2021年进行，采用加州乳腺炎试验和体细胞计数对马图拉地区及周边不同的gaushalas和奶牛场进行乳腺炎筛查。阳性样本通过细菌、生化和分子检测以及抗微生物药物耐药性谱进行了进一步检测。结果:我们的研究发现在亚临床样品中有大量的金黄色葡萄球菌，存在mecA基因提示MRSA。金黄色葡萄球菌的公共卫生重要性和对抗生素的新耐药性要求对MRSA分离株进行定期监测和有效使用抗菌药物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of dairying, foods & home sciences

CiteScore

0.70

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0.00%

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