Establishing an effective protocol for micropropagation of mullberry (Morus nigra L.)

Abstract

Murashige and Skoog (MS) medium supplemented with plant growth regulators composed of different concentrations were used in vitro rapid and mass multiplication of nodal explants obtained from shoots of black mulberry (Morus nigra L.) seedlings grown in a fully controlled greenhouse. Eighteen different concentration of 6-benzylaminopurin, thidiazuron (TDZ), kinetin (KN) GA3 and naphthalene acetic acid (NAA) as plant growth regulators were used. In contrast to expectation, combinations of (TDZ 1.0 mg L–1 + GA3 0.25 mg L–1) and (TDZ 2.0 mg L–1 + GA3 0.25 mg L–1) were found to give positive results in shoot proliferation and plant formation at the end of the study. In the rooting study (NAA 0.5 mg L–1 + KN 0.1 mg L–1) and (NAA 0.5 mg L–1 + KN 0.2 mg L–1) treatment showed positive results.