Dual-targeted drug delivery system based on dopamine functionalized human serum albumin nanoparticles as a carrier for methyltestosterone drug

IF 1.4 Q4 NANOSCIENCE & NANOTECHNOLOGY
Tayebeh Noori, S. Kashanian, R. Rafipour, K. Mansouri, M. Nazari
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引用次数: 1

Abstract

Objective(s): This study aims to enhance 17 a-methyltestosterone loaded human serum albumin nanoparticles (MT-HSA NPs) bioavailability through a desolvation technique. Dopamine (DA) molecules were conjugated on the surface of MT-HSA NPs and have the potential to act as tiny proper ligands in a unique treatment system to cope with cancer in which drug will be transmitted to the cancer area. Herein, we used HSA as an adaptable carrier of anticancer agents for methyltestosterone transport to the tumor site via DA D1-D5 receptors. In the present study, sonication of MT-HSA solution was carried out before the desolvation procedure to increase the drug loading and entrapment efficiency. Materials and Methods: Various parameters were optimized to characterize NPs including morphology, size, zeta potential, polydispersity index, drug release profile, and entrapment efficiency. Results: Under the optimum conditions of HSA and drug (1:41), at pH 9, results demonstrate sizes of 69 nm and 82 nm for MT-HSA and MT-HSA-DA NPs respectively. For MT-HSA NPs, the polydispersity index was found to be 0.3 and the average drug loading and encapsulation efficiency were 14% and 91% respectively. Anticancer activity and the release of drug was investigated through MCF-7 breast cancer cell line. Results show that targeted NPs are more effective than non-targeted NPs. Conclusion: According to these studies, the therapeutic effects against various diseases such as cancers increase through cellular targeting property of a biocompatible drug delivery system. This is the first report for methyltestosterone delivery to breast cancer cells based on HSA NPs.
多巴胺功能化人血清白蛋白纳米粒子作为甲基睾酮药物载体的双靶向给药系统
目的:本研究旨在通过去溶剂化技术提高17α-甲基睾酮负载的人血清白蛋白纳米颗粒(MT-HSA NPs)的生物利用度。多巴胺(DA)分子结合在MT-HSA NP的表面上,并有可能在独特的治疗系统中作为微小的适当配体来应对癌症,在这种治疗系统中,药物将传播到癌症区域。在此,我们使用HSA作为抗癌剂的适应性载体,通过DA D1-D5受体将甲基睾酮转运到肿瘤部位。在本研究中,在去溶剂化程序之前对MT-HSA溶液进行超声处理,以提高药物负载和包封效率。材料和方法:优化各种参数来表征纳米颗粒,包括形态、大小、ζ电位、多分散指数、药物释放谱和包封效率。结果:在HSA和药物(1:41)的最佳条件下,在pH9下,MT-HSA和MT-HSA-DA NPs的尺寸分别为69nm和82nm。对于MT-HSA NP,发现多分散性指数为0.3,平均载药量和包封效率分别为14%和91%。通过MCF-7乳腺癌症细胞系研究其抗癌活性和药物的释放。结果表明,靶向NP比非靶向NP更有效。结论:根据这些研究,通过生物相容性药物递送系统的细胞靶向特性,可以提高对癌症等各种疾病的治疗效果。这是第一份基于HSA NP的甲基睾酮递送到乳腺癌症细胞的报告。
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来源期刊
Nanomedicine Journal
Nanomedicine Journal NANOSCIENCE & NANOTECHNOLOGY-
CiteScore
3.40
自引率
0.00%
发文量
0
审稿时长
12 weeks
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