Low-cost automated pipetting system using a single board computer and 3D-printing

IF 1.3 4区工程技术 Q4 CHEMISTRY, ANALYTICAL

Instrumentation Science & Technology Pub Date : 2022-10-03 DOI:10.1080/10739149.2022.2129677

Nagarjuna Boppana, Robyn A. Snow, Paul S. Simone, G. L. Emmert, Michael A. Brown

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引用次数: 1

Abstract

Abstract A low-cost, automated pipetting system (auto pipet) was developed to deliver microliter volumes of aqueous solutions. This system was constructed using a single board computer, 3 D-printed parts, stepper motors, and other commercially available components. The performance of the auto pipet was found to be comparable or better than manual pipetting and a commercially available liquid handling system. Inter-day, intra-day, inter-instrumental, and intra-instrumental validation studies were performed to evaluate the accuracy and precision of liquid delivery using the auto pipet. Accuracy was determined to be from 98 to 102% with the precision, represented as the relative standard deviation, to be less than 3%. The auto pipet was further evaluated by the preparation of calibration and check standards for the determination of method detection limit, accuracy, and precision for trihalomethanes using a commercially available monitoring system. The auto pipet was also used as a digital buret to perform water hardness and water alkalinity titrations with promising results. Graphical Abstract

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低成本自动化移液系统，使用单板计算机和3d打印

摘要开发了一种低成本的自动移液系统（自动移液管），用于输送微升体积的水溶液。该系统使用单板计算机构建，3 D打印零件、步进电机和其他商用部件。发现自动移液器的性能与手动移液器和市售的液体处理系统相当或更好。进行了日间、日间、仪器间和仪器内验证研究，以评估使用自动移液管输送液体的准确性和精密度。准确度被确定为98%至102%，精密度（以相对标准偏差表示）小于3%。通过使用市售监测系统制备用于测定三卤甲烷的方法检测限、准确度和精密度的校准和检查标准，对自动移液管进行了进一步评估。自动移液管也被用作数字滴定管来进行水硬度和水碱度的滴定，结果很有希望。图形摘要

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来源期刊

Instrumentation Science & Technology 工程技术-分析化学

CiteScore

3.50

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Instrumentation Science & Technology is an internationally acclaimed forum for fast publication of critical, peer reviewed manuscripts dealing with innovative instrument design and applications in chemistry, physics biotechnology and environmental science. Particular attention is given to state-of-the-art developments and their rapid communication to the scientific community. Emphasis is on modern instrumental concepts, though not exclusively, including detectors, sensors, data acquisition and processing, instrument control, chromatography, electrochemistry, spectroscopy of all types, electrophoresis, radiometry, relaxation methods, thermal analysis, physical property measurements, surface physics, membrane technology, microcomputer design, chip-based processes, and more. Readership includes everyone who uses instrumental techniques to conduct their research and development. They are chemists (organic, inorganic, physical, analytical, nuclear, quality control) biochemists, biotechnologists, engineers, and physicists in all of the instrumental disciplines mentioned above, in both the laboratory and chemical production environments. The journal is an important resource of instrument design and applications data.