Hematotoxic, oxidative and genotoxic damage in rainbow trout (Oncorhynchus mykiss) after exposure to 3-benzoylpyridine

IF 2.8 4区医学 Q2 TOXICOLOGY

Toxicology Mechanisms and Methods Pub Date : 2022-03-10 DOI:10.1080/15376516.2022.2049413

Veysel Parlak, Bunyamin Ozgeris, A. Uçar, A. ÇİLİNGİR YELTEKİN, F. B. Ozgeris, O. Caglar, G. Alak, H. Turkez, M. Atamanalp

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引用次数: 2

Abstract

Abstract Pyridine is a basic heterocyclic organic compound. The pyridine ring is present in many important compounds, including agricultural chemicals, medicines and vitamins. Due to their widespread industrial use, bioaccumulation and non-target toxic effects are being considered as a great risk to human and environmental health. In this study, we aimed to evaluate the hematological, oxidative and genotoxic damage potentials by different concentrations (1, 1.5, and 2 g/L) of the ketone 3-Benzoylpyridine (3BP) on rainbow trout (Oncorhynchus mykiss). Alterations in the biomarker levels of oxidative DNA damage (8-hydroxy-2′-deoxyguanosine (8-OHdG)), apoptosis (Caspase-3), malondialdehyde (MDA) as well as antioxidant enzyme activities including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), myeloperoxidase (MPO), paraoxonase (PON), and arylesterase (AR) were assessed in brain, liver, gill and blood tissues. Acetylcholinesterase (AChE) activity was also determined in brain tissue. In addition, we analyzed micronucleus (MN) rates and hematological indices of total erythrocyte count (RBC), total leukocyte count (WBC), hemoglobin (Hb), hematocrit (Hct), total platelet count (PLT), mean cell hemoglobin concentration (MCHC), mean cell hemoglobin (MCH), and mean cell volume (MCV) in blood. LC50-96h value of 3BP was calculated as 5.2 g/L from the data obtained. A significant decrease in brain AChE activity was determined in clear time and dose dependent manners. While SOD, CAT, GPx, PON, and AR levels were decreased, MDA, MPO, 8-OHdG and Caspase-3 levels were increased in all tissues (p < 0.05). Again, the 3BP led to increases of MN formation at all applied concentrations in the rates of between 45.4 and 72.7%. Significant differences (p < 0.05) were found out in between all studied hematology parameters between 3BP-exposed and the control fish. In conclusion, ours study firstly indicated that the treatment doses of 3BP induced distinct hematological and oxidative alterations as well as genotoxic damage in rainbow trout.

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虹鳟暴露于3-苯甲酰吡啶后的血毒、氧化和基因毒性损伤

摘要吡啶是一种碱性杂环有机化合物。吡啶环存在于许多重要的化合物中，包括农用化学品、药物和维生素。由于其广泛的工业用途，生物累积和非目标毒性作用被认为是对人类和环境健康的巨大风险。在本研究中，我们旨在评估不同浓度（1、1.5和2 g/L）酮3-苯甲酰基吡啶（3BP）对虹鳟（Oncorhynchus mykiss）的作用。评估了大脑、肝脏、鳃和血液组织中氧化性DNA损伤（8-羟基-2′-脱氧鸟苷（8-OHdG））、细胞凋亡（Caspase-3）、丙二醛（MDA）以及抗氧化酶活性（包括超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPX）、髓过氧化物酶（MPO）、对氧酶（PON）和芳基酯酶（AR））的生物标志物水平的变化。还测定了脑组织中乙酰胆碱酯酶（AChE）的活性。此外，我们还分析了血液中红细胞总数（RBC）、白细胞总数（WBC）、血红蛋白（Hb）、红细胞比容（Hct）、血小板总数（PLT）、平均细胞血红蛋白浓度（MCHC）、平均血红蛋白（MCH）和平均细胞体积（MCV）的微核率和血液学指标。3BP的LC50-96h值计算为5.2 g/L。以明确的时间和剂量依赖性方式测定了脑AChE活性的显著降低。SOD、CAT、GPx、PON和AR水平降低，MDA、MPO、8-OHdG和Caspase-3水平升高（p < 0.05）。同样，3BP在所有施用浓度下都导致MN形成的增加，增加率在45.4%和72.7%之间 < 0.05）在暴露于3BP和对照鱼之间的所有研究的血液学参数之间发现。总之，我们的研究首次表明，3BP的治疗剂量对虹鳟产生了明显的血液学和氧化改变以及遗传毒性损伤。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Toxicology Mechanisms and Methods TOXICOLOGY-

自引率

3.10%

发文量

期刊介绍： Toxicology Mechanisms and Methods is a peer-reviewed journal whose aim is twofold. Firstly, the journal contains original research on subjects dealing with the mechanisms by which foreign chemicals cause toxic tissue injury. Chemical substances of interest include industrial compounds, environmental pollutants, hazardous wastes, drugs, pesticides, and chemical warfare agents. The scope of the journal spans from molecular and cellular mechanisms of action to the consideration of mechanistic evidence in establishing regulatory policy. Secondly, the journal addresses aspects of the development, validation, and application of new and existing laboratory methods, techniques, and equipment. A variety of research methods are discussed, including: In vivo studies with standard and alternative species In vitro studies and alternative methodologies Molecular, biochemical, and cellular techniques Pharmacokinetics and pharmacodynamics Mathematical modeling and computer programs Forensic analyses Risk assessment Data collection and analysis.