Characterization of life-history parameters of an Anopheles funestus (Diptera: Culicidae) laboratory strain

IF 1.4 3区农林科学 Q2 ENTOMOLOGY

Journal of Vector Ecology Pub Date : 2021-06-01 DOI:10.52707/1081-1710-46.1.24

M. P. Zengenene, G. Munhenga, Glory Chidumwa, L. Koekemoer

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引用次数: 9

Abstract

ABSTRACT: The colonization of the African malaria vector Anopheles funestus has been hampered by inadequate knowledge of its mating and development under laboratory conditions. Life-tables are routinely used to provide baseline biological characteristics needed for colonization. This study characterized age-specific life-table attributes of an existing An. funestus laboratory strain to gain insight into factors that are critical for its colonization. To achieve this, the An. funestus laboratory strain was reared from eggs to adulthood under standard insectary conditions, monitoring and characterizing each developmental stage. The mean insemination rate of females was 74.8% with an average egg load of 67.1 eggs/female and a mean fertility of 86.7%. The mean developmental time from 1st instar larvae (L1) to pupation was 16.4 days. The mean proportion of L1 that survived to pupation was 72.9%. On average, 78.8% of the pupae successfully eclosed as adults. The median longevity for adult males and females was 44 and 28 days, respectively. This work constitutes the first report on life-table characterization of an An. funestus strain. The larval developmental time was within the range reported for wild An. funestus while adult longevity was higher compared to survivorship observed in wild populations. These data demonstrate that the colonized An. funestus strain has potential to be re-colonized under standard insectary conditions. The study provides base-line information for further studies on identifying critical parameters for the maintenance of An. funestus under artificial conditions.

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一种富氏按蚊(双翅目:库蚊科)实验室菌株生活史参数的表征

摘要：由于对非洲疟原虫的交配和实验室条件下的发育缺乏足够的了解，非洲疟原虫的定殖一直受到阻碍。生命表通常用于提供定殖所需的基线生物学特征。这项研究表征了一种现有的an.funcestus实验室菌株的年龄特异性生命表属性，以深入了解对其定殖至关重要的因素。为了实现这一点，在标准的昆虫条件下，从卵到成年，对每个发育阶段进行监测和表征。雌虫平均受精率为74.8%，平均产卵量为67.1卵/雌，平均生育率为86.7%。从1龄幼虫（L1）到化蛹的平均发育时间为16.4天。L1的平均羽化率为72.9%，平均有78.8%的成虫羽化成功。成年男性和女性的中位寿命分别为44天和28天。这项工作构成了首次报告的生命表特征的安。funestus菌株。幼虫的发育时间在野生An.funcestus报告的范围内，而与野生种群中观察到的存活率相比，成虫的寿命更高。这些数据表明，在标准昆虫条件下，定殖的An.funcestus菌株具有重新定殖的潜力。这项研究为进一步研究确定人工条件下悬铃木维护的关键参数提供了基线信息。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Vector Ecology 生物-昆虫学

CiteScore

2.60

自引率

5.90%

发文量

审稿时长

6-12 weeks

期刊介绍： The Journal of Vector Ecology is an international journal published by the Society for Vector Ecology. It is concerned with all aspects of the biology, ecology, and control of arthropod and vertebrate vectors and the interrelationships between the vectors and the agents of disease that they transmit. The journal publishes original research articles and scientific notes, as well as comprehensive reviews of vector biology based on presentations at Society meetings. All papers are reviewed by at least two qualified scientists who recommend their suitability for publication. Acceptance of manuscripts is based on their scientific merit and is the final decision of the editor, but these decisions may be appealed to the editorial board. The journal began publishing in 1974 and now publishes on-line only.