Correlation between HBV large protein and HBV replication in patients with chronic hepatitis B

Q4 Immunology and Microbiology

中华微生物学和免疫学杂志 Pub Date : 2020-02-29 DOI:10.3760/CMA.J.CN112309-20190720-00220

Zhenzi Wang, Tie-shan Wang

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Abstract

Objective To investigate the correlation of serum hepatitis B virus large protein (HBV-LP), HBV-DNA, and Pre S1 antigen (Pre S1-Ag) with HBV replication. Methods Serum samples were collected from 650 patients with chronic hepatitis B (CHB) who were treated in Beijing Friendship Hospital from March 2017 to March 2019. Serum HBV-LP and Pre S1-Ag were detected by enzyme-linked immunosorbent assay (ELISA). HBV markers (HBV-M) were measured using chemiluminescent microparticle immunoassay (CMIA). Quantitative real-time PCR (qRT-PCR) was used to detect HBV-DNA. The positive detection rates of HBV-DNA, HBV-LP and Pre S1-Ag were calculated and compared, and the correlation of HBV-LP (S/CO value) and hepatitis B surface antigen (HBsAg, log10 IU/ml) with HBV-DNA(log10 IU/ml)was analyzed. Results In the 650 CHB patients, the positive rates of HBV-DNA, HBV-LP and Pre S1-Ag were 65.4% (425/650), 79.2% (515/650) and 43.1% (280/650), respectively (P<0.01). The positive rates of HBV-DNA and HBV-LP in 243 HBeAg-positive patients were 93.0% (226/243) and 94.6% (230/243), and no significant difference was found between them (P=0.45). However, there was significant difference between the positive rates of HBV-DNA and HBV-LP in 407 patients negative for HBeAg [48.9% (199/407) vs 70.0% (285/407), P<0.01]. The positive rates of HBV-DNA and HBV-LP in HBsAg-, HBeAg- and HBcAb-positive groups were 92.8% (206/222) and 94.1% (209/222), which showed no significant difference (P=0.56). In HBsAg-, HBeAb- and HBcAb-positive groups, the positive rates of HBV-DNA and HBV-LP were 45.4% (124/273) and 69.9% (191/273) (P<0.01). The detection rate of HBeAg was lower than that of HBV-LP significantly in both HBV-DNA-positive and HBV-DNA-negative groups (P<0.01). With the increasing of HBV-DNA load, the S/CO value and the positive rate of HBV-LP increased significantly (P<0.05). Conclusions HBV-LP had a good correlation with HBV-DNA load as compared with Pre S1-Ag, HBeAg and HBsAg. HBV-LP in combination with HBV-M might be used as predictive markers that could efficiently reflect the status of HBV replication. Key words: Hepatitis B virus; DNA; Large protein; Pre S1 antigen; HBV markers

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慢性乙型肝炎患者HBV大蛋白与HBV复制的相关性

目的探讨血清乙型肝炎病毒大蛋白（HBV-LP）、HBV-DNA、前S1抗原（Pre-S1Ag）与HBV复制的相关性。方法收集2017年3月至2019年3月在北京友谊医院就诊的650例慢性乙型肝炎患者的血清样本。采用酶联免疫吸附试验（ELISA）检测血清HBV-LP和Pre-S1Ag。用化学发光微粒免疫测定法（CMIA）测定HBV标志物（HBV-M）。采用实时定量聚合酶链式反应（qRT-PCR）检测HBV-DNA。计算并比较了HBV-DNA、HBV-LP和Pre-S1Ag的阳性检出率，分析了HBV-LP（S/CO值）和乙型肝炎表面抗原（HBsAg，log10IU/ml）与HBV-DNA（log10IUml）的相关性。结果在650例慢性乙型肝炎患者中，HBV-DNA、HBV-LP和Pre-S1Ag的阳性率分别为65.4%（425/650）、79.2%（515/650）和43.1%（280/650）（P<0.01），407例HBeAg阴性患者的HBV-DNA和HBV-LP阳性率有显著性差异[48.9%（199/407）vs 70.0%（285/407），P<0.01，HBV-DNA和HBV-LP的阳性率分别为45.4%（124/273）和69.9%（191/273）（P<0.01），S／CO值和HBV-LP阳性率显著升高（P＜0.05）。HBV-LP与HBV-M联合应用可作为有效反映HBV复制状态的预测标志物。关键词：乙型肝炎病毒；DNA；大蛋白；S1前抗原；HBV标志物

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来源期刊

中华微生物学和免疫学杂志 Immunology and Microbiology-Virology

CiteScore

0.50

自引率

0.00%

发文量

6906

期刊介绍： Chinese Journal of Microbiology and Immunology established in 1981. It is one of the series of journal sponsored by Chinese Medical Association. The aim of this journal is to spread and exchange the scientific achievements and practical experience in order to promote the development of medical microbiology and immunology. Its main contents comprise academic thesis, brief reports, reviews, summaries, news of meetings, book reviews and trends of home and abroad in this field. The distinguishing feature of the journal is to give the priority to the reports on the research of basic theory, and take account of the reports on clinical and practical skills.