The chitinase activity of oil palm (Elaeis guineensis Jacq.) roots against fungal endophytes and pathogenic Ganoderma boninense

Q3 Agricultural and Biological Sciences

Plant Omics Pub Date : 2017-12-20 DOI:10.21475/POJ.10.05.17.PNE853

Yurnaliza, R. Esyanti, A. Susanto, I. Aryantha

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引用次数: 8

Abstract

Application of fungal endophytes can be an alternative to control basal stem rot disease in oil palm, caused by 'Ganoderma boninense'. Chitinase is a type of defensive protein synthesized by plants in response to biotic factors. The purpose of this study was to analyze the chitinase activity of oil palm as a defensive mechanism to fungal endophytes and pathogenic 'G. boninense'. Four species of fungal endophytes, 'Trichoderma harzianum' MTP 10 (Th-MTP10), Trichoderma longibrachiatum KBA 31 (Tl-KBA 31), 'Lasiodiplodia venezuelensis' MJP 28 (Lv-MJP 28), 'Dothidiomycetes' sp. MTD 29 (Dt-MTD 29) and one species of fungal pathogen 'G. boninense' and with their each cell wall suspension were introduced to oil palm plantlets in axenic condition. Chitinase activity was observed from the root of oil palm plantlets inoculated with both living cell and cell wall suspension of endophytic fungi and pathogenic 'G. boninense'. Results showed that chitinase activities varied in each fungal treatment and were significantly differed from control. Fungal cell wall elicitors were able to significantly induce chitinase activity after 1 week post treatment (wpt). Statistically, only the chitinase activity from fungal endophyte Lv-MJP 28 was significantly higher from others for 8 and 12 days. Pre-treatment of oil palm plantlet with fungal cell wall suspension for 1 wpt could induce the chitinase activity higher than control when, oil palm infected with fungal pathogen of 'Ganoderma boninense'.

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油棕(Elaeis guineensis Jacq.)根对真菌内生菌和致病性灵芝几丁质酶活性的研究

应用真菌内生菌可以作为控制油棕根茎腐病的一种替代方法，这种病是由'灵芝'引起的。几丁质酶是植物为应对生物因子而合成的一类防御蛋白。本研究的目的是分析油棕几丁质酶活性对真菌内生菌和致病性“G. boninense”的防御机制。将4种内生真菌‘哈兹木霉’MTP10 (Th-MTP10)、‘长直链木霉’KBA 31 (Tl-KBA 31)、‘Lasiodiplodia dvelensis’MJP 28 (lm -MJP 28)、‘Dothidiomycetes’sp. MTD 29 (Dt-MTD 29)和1种真菌病原‘G. boninense’及其细胞壁悬悬液引入油棕植株，并对其进行了培养。用内生真菌和致病性“G. boninense”活细胞和细胞壁悬浮液分别接种油棕植株，观察其根部几丁质酶活性。结果表明，不同真菌处理下几丁质酶活性差异显著，与对照差异显著。真菌细胞壁激发剂在处理后1周显著诱导几丁质酶活性。统计上，只有真菌内生菌Lv-MJP 28的几丁质酶活性在8天和12天内显著高于其他真菌。用真菌细胞壁悬浮液预处理油棕幼苗1 wpt后，油棕感染“博南灵芝”真菌病原菌后，其几丁质酶活性高于对照。

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来源期刊

Plant Omics 生物-植物科学

CiteScore

1.30

自引率

0.00%

发文量

审稿时长

6 months

期刊介绍： Plant OMICS is an international, peer-reviewed publication that gathers and disseminates fundamental and applied knowledge in almost all area of molecular plant and animal biology, particularly OMICS-es including: Coverage extends to the most corners of plant and animal biology, including molecular biology, genetics, functional and non-functional molecular breeding and physiology, developmental biology, and new technologies such as vaccines. This journal also covers the combination of many areas of molecular plant and animal biology. Plant Omics is also exteremely interested in molecular aspects of stress biology in plants and animals, including molecular physiology.