Myelin enhancement of Multiple sclerosis model with gold nanoparticles into the corpus callosum

IF 1.4 Q4 NANOSCIENCE & NANOTECHNOLOGY

Nanomedicine Journal Pub Date : 2019-06-15 DOI:10.22038/NMJ.2019.06.0000010

M. Khosravi, M. Karami, M. J. Nadoushan, A. Hajnorouzi

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引用次数: 1

Abstract

Objective(s): With no substantial cost, we injected L-arginine into the rat’s corpus callosum (CC) to create animal model of multiple sclerosis (MS) and investigated the pre-injection effect of gold nanoparticles (GNPs). Materials and Methods: Adult male Wistar rat (250-300 g) was surgically cannulated at the CC, and after recovery it was injected L-arginine (3-200 µg/rat, intra-CC) once daily for 3 to 5 consecutive days. GNPs (0.001-0.01 µg/rat, intra-CC) were injected alone or prior to the L-arginine using the same procedure. Control group solely received saline (1 µL/rat, intra-CC). Brain was studied with luxol fast blue. Weight change was also analyzed via the analysis of variance (ANOVA). Results: L-arginine significantly induced (p< 0.05) a reduction in the fiber density while the neurons increased (p< 0.05). Single GNPs reduced (p< 0.05) the fiber and neuron densities; however, pre-injection of NPs caused myelinated fibers and uniform density of neurons. Conclusion: The L-arginine may trigger demyelination by pro-inflammatory nitric oxide (NO), and the GNPs may improve this effect.

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胼胝体金纳米颗粒对多发性硬化症模型髓磷脂的增强作用

目的：在不需要大量费用的情况下，我们将L-精氨酸注射到大鼠胼胝体（CC）中以建立多发性硬化（MS）动物模型，并研究了金纳米粒子（GNPs）的注射前效应。材料和方法：成年雄性Wistar大鼠（250-300g）通过手术在CC处插管，恢复后每天注射L-精氨酸（3-200µg/只，CC内）一次，连续3-5天。GNP（0.001-0.01µg/大鼠，CC内）单独注射或在使用相同程序注射L-精氨酸之前注射。对照组仅接受生理盐水（1µL/大鼠，CC内）。用luxol快蓝对大脑进行了研究。体重变化也通过方差分析（ANOVA）进行分析。结果：L-精氨酸可显著降低（p＜0.05）纤维密度，而神经元数量增加（p＜0.01），单次GNP可降低（p＞0.05）纤维和神经元密度；然而，NPs的预注射引起有髓鞘纤维和神经元的均匀密度。结论：L-精氨酸可能通过促炎性一氧化氮（NO）触发脱髓鞘，GNPs可能改善这种作用。

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来源期刊

Nanomedicine Journal NANOSCIENCE & NANOTECHNOLOGY-

CiteScore

3.40

自引率

0.00%

发文量

审稿时长

12 weeks