Aggregates of α-synuclein in brain tissue homogenates measured by newly designed Multimer-PAGE techniques

Q4 Biochemistry, Genetics and Molecular Biology

Journal of Cellular Neuroscience and Oxidative Stress Pub Date : 2019-06-27 DOI:10.37212/JCNOS.584670

J. Saleh

引用次数: 1

Abstract

Atypical aggregation of α-synuclein in the brain is linked to Parkinson’s disease (PD) progression. Mutimers of α-synuclein may be important for the biological function of α-synuclein. Currently, reliable methods to compare α-synuclein multimer abundance in biological samples are limited and sophisticated. A new technique termed “multimer-PAGE,” was designed by Killinger and Moszczynska, which combines in-gel chemical cross-linking with several common electrophoretic techniques to measure the multimerization of α-synuclein in complex biological samples of brain tissue lysates. Using this technique, it was possible to compare the ratio of α-synuclein multimers between brain tissue samples without the need for specialized equipment.

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新设计的Multimer PAGE技术测定脑组织匀浆中α-突触核蛋白的聚集

大脑中α-突触核蛋白的非典型聚集与帕金森病（PD）的进展有关。α-突触核蛋白的多聚体可能对α-突触蛋白的生物学功能有重要意义。目前，比较生物样品中α-突触核蛋白多聚体丰度的可靠方法是有限和复杂的。Killinger和Moszczynska设计了一种名为“多聚体PAGE”的新技术，该技术将凝胶内化学交联与几种常见的电泳技术相结合，以测量脑组织裂解物的复杂生物样品中α-突触核蛋白的多聚化。使用这项技术，可以在不需要专门设备的情况下比较脑组织样本中α-突触核蛋白多聚体的比例。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Cellular Neuroscience and Oxidative Stress Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

1.10

自引率

0.00%

发文量

期刊介绍： Journal of Cellular Neuroscience and Oxidative Stress isan online journal that publishes original research articles, reviews and short reviews on themolecular basisofbiophysical,physiological and pharmacological processes thatregulate cellular function, and the control or alteration of these processesby theaction of receptors, neurotransmitters, second messengers, cation, anions,drugsor disease. Areas of particular interest are four topics. They are; 1. Ion Channels (Na+-K+Channels, Cl– channels, Ca2+channels, ADP-Ribose and metabolism of NAD+,Patch-Clamp applications) 2. Oxidative Stress (Antioxidant vitamins, antioxidant enzymes, metabolism of nitric oxide, oxidative stress, biophysics, biochemistry and physiology of free oxygen radicals) 3. Interaction Between Oxidative Stress and Ion Channels in Neuroscience (Effects of the oxidative stress on the activation of the voltage sensitive cation channels, effect of ADP-Ribose and NAD+ on activation of the cation channels which are sensitive to voltage, effect of the oxidative stress on activation of the TRP channels in neurodegenerative diseases such Parkinson’s and Alzheimer’s diseases) 4. Gene and Oxidative Stress (Gene abnormalities. Interaction between gene and free radicals. Gene anomalies and iron. Role of radiation and cancer on gene polymorphism)