Study of the solubilisation process of bacterial model membranes induced by DDAO

European Pharmaceutical Journal Pub Date : 2021-01-01 DOI:10.2478/afpuc-2020-0019

K. Želinská, J. Gallová

引用次数: 0

Abstract

Abstract Solubilisation of two bacterial model membranes induced by N,N-dimethyl-1-dodecanamine-N-oxide (DDAO) was studied. The first model membrane consisted of a mixture of palmitoyloleoylphosphatidylethanolamine (POPE) and palmitoyloleoylphosphatidylglycerol (POPG) in a molar ratio 0.6:0.4 mol/mol, and a second model membrane was enriched with tetraoleoylcardiolipin (TOCL) with a composition POPE-POPG-TOCL = 0.67:0.23:0.1 mol/mol/mol. Solubilisation of these model membranes was studied by static light scattering (nephelometry). Effective ratio Re (the amount of DDAO integrated into the bilayer to the amount of lipid) at different steps of the solubilisation process was determined. The molar partition coefficient of DDAO was calculated – in case of the POPE-POPG membrane, Kp = 5,300 ± 400, for the POPE-POPG-TOCL membrane, Kp = 6,500 ± 500.

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DDAO诱导细菌模型膜溶解过程的研究

摘要研究了N,N-二甲基-1-十二胺-N-氧化物(DDAO)对两种细菌模型膜的增溶作用。第一种模型膜由棕榈酰油酰基磷脂酰乙醇胺(POPE)和棕榈酰油酰基磷脂酰甘油(POPG)组成，摩尔比为0.6:0.4 mol/mol;第二种模型膜富含四油酰基心磷脂(TOCL)，组成为POPE-POPG-TOCL = 0.67:0.23:0.1 mol/mol/mol。通过静态光散射(浊度法)研究了这些模型膜的增溶作用。测定了溶解过程中不同步骤的有效比Re(整合到双分子层中的DDAO的量与脂质的量)。计算了DDAO的摩尔分配系数，对于POPE-POPG膜，Kp = 5,300±400，对于POPE-POPG- tocl膜，Kp = 6,500±500。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

European Pharmaceutical Journal Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)

CiteScore

0.60

自引率

0.00%

发文量

期刊介绍： European Pharmaceutical Journal publishes only original articles not previously published and articles that are not being considered or have not been submitted for publication elsewhere. If parts of the results have been published as conference abstract or elsewhere, it should be stated in references. The ethical standards of the Helsinki-Tokio Declaration should be kept. This should be mentioned in the Methods of manuscript. Reviews are published only on request. Authors, whose submitted research work was performed with the support of a company, should indicate this in Conflict of Interest.