Evaluation Efficiency of Different Isolate of Actinomycetes for Control of Cucumber Seedling Damping-off Disease Caused by Rhizoctonia solani (Khun)

Q4 Agricultural and Biological Sciences

Arab Journal of Plant Protection Pub Date : 2021-12-01 DOI:10.22268/ajpp-39.4.281288

Mohamad Amer Fayyadh, Lina K. Awad

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Abstract

Fayyadh, M.A. and L.K. Awad. 2021. Evaluation Efficiency of Different Isolate of Actinomycetes for Control of Cucumber Seedling Damping-off Disease Caused by Rhizoctonia solani (Khun). Arab Journal of Plant Protection, 39(4): 281-288. https://doi.org/10.22268/AJPP-39.4.281288 This study was conducted in Plant Protection Department, College of Agriculture, University of Basrah during the period 2017-2018 aimed to isolate and identify Actinomycetes from different environmental sources and evaluate their efficiency to control cucumber damping off disease caused by Rhizoctonia solani. 28 isolates of Actinomycetes were isolated from different sources from the Basrah region. All such isolates were gram positive, amylase and catalase positive and they had branched hyphae. Molecular identification following amplification of 16sRNA confirmed that Actinomycetes isolate No 6 isolated from soil had a similarity of 99% with Streptomyces griseus, whereas the isolate No 66 isolated from date palm roots had a similarity of 99% with Brevibacterium celere. The nucleotide sequence of the two isolates has been deposited at NCBI with Genbank accession number LC501385.1 for S. griseus and LC501386.1 for B. celere. The dual culture technique showed that Actinomycetes isolates S. griseus and B. celere had high antagonistic activity against Rhizoctonia solani, which produced inhibition zones of 7 and 15 mm in dimeter, respectively. On the other hand, volatile compoundsreleased from S. griseus and B. celere inhibited the growth of R. solani by 68 and 81.5%, respectively. Pot experiment showed that all actinomycetes isolates significantly reduced cucumber seedling damping–off incidence caused by R. solani. Keywords: Actinomycetes, Rhizoctonia solani, Cucumber, Biological Control

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不同放线菌分离株防治黄瓜枯枯病的效果评价

Fayyadh, M.A.和L.K. Awad. 2021。不同放线菌分离株防治黄瓜枯枯病效果评价植物保护学报，39(4):281-288。https://doi.org/10.22268/AJPP-39.4.281288本研究于2017-2018年在巴士拉大学农学院植物保护系进行，目的是分离和鉴定来自不同环境来源的放线菌，并评估其对黄瓜枯丝核菌病的防治效果。从巴士拉地区不同来源分离到28株放线菌。所有分离株革兰氏阳性，淀粉酶和过氧化氢酶阳性，菌丝分枝。16sRNA扩增后的分子鉴定表明，从土壤中分离得到的放线菌No 6与灰色链霉菌相似度为99%，而从枣椰树根中分离得到的放线菌No 66与celere短杆菌相似度为99%。这两株菌株的核苷酸序列已存入NCBI, S. griseus和B. celere的Genbank登录号分别为LC501385.1和LC501386.1。双重培养技术表明，分离的放线菌S. griseus和B. celere对茄枯丝核菌具有较高的拮抗活性，分别产生直径为7 mm和15 mm的抑菌带。另一方面，稻瘟病菌和白孢杆菌释放的挥发性化合物对茄枯病菌的抑制作用分别为68%和81.5%。盆栽试验结果表明，所有放线菌分离株均能显著降低番茄枯萎病对黄瓜幼苗的影响。关键词:放线菌，番茄丝核菌，黄瓜，生物防治

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来源期刊

Arab Journal of Plant Protection Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

0.40

自引率

0.00%

发文量

期刊介绍： The Arab Journal of Plant Protection is an open access journal included in CABI, AGRIS and Google Scholar data bases and indexed by Scopus. The journal’s aim is the promotion of plant health for crops grown in the Arab and Near East region and for safe food production and transfer of new knowledge on plant pests and their sustainable management. The journal deals with all scientific