Epidemiological study on respiratory syncytial virus-caused respiratory infection in children and preliminary analysis of changes in immune function

Q4 Immunology and Microbiology

中华微生物学和免疫学杂志 Pub Date : 2020-02-29 DOI:10.3760/CMA.J.CN112309-20190809-00248

Wei-wei Guo, Xiaotong Yan, Z. Feng, Huirong Huang, Le Wang, Guixia Li, Yong Wu

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引用次数: 1

Abstract

Objective To analyze the epidemiological characteristics of respiratory syncytial virus (RSV)-caused respiratory diseases in children in Hebei Province and the changes in peripheral blood lymphocyte subsets and inflammatory indexes. Methods A total of 9 491 sputum specimens and 9 491 paired peripheral blood specimens were collected from children admitted to Hebei Children′s Hospital for respiratory infection in 2018. RSV-positive sputum specimens were screened by multiple detection reagents for 13 kinds of respiratory pathogens. Flow cytometry was uses to detect T and B lymphocytes and NK cells in peripheral blood samples of randomly screened RSV-positive children. Procalcitonin (PCT) was measured by Roche E411 luminescence analyzer. Hypersensitive C-reactive protein (hs-CRP) was detected by Roche Cobas 8000 C701 biochemical analyzer. White blood cells (WBC) were measured by Sysmex XN-BN3 hematometer. Results The positive rate of RSV in children with respiratory diseases was 13.08% in Hebei Province in 2018. There were significant differences in RSV-positive rates among different age groups (χ2=479.297 6, P<0.000 1). The positive rate of RSV decreased gradually with age (χ2=-20.282 7, P<0.000 1) and was higher in male than in female (χ2=34.552 7, P<0.000 1). The incidence of co-infection of RSV with other respiratory pathogens was 29.49% (366/1 241), mainly caused by human rhinovirus (HRV, 150/1 241) and adenovirus (ADV, 40/1 241). The main epidemic seasons of RSV infection were winter and spring. The epidemic trends of simple RSV infection and co-infection were consistent. There were significant differences in inflammatory indexes, WBC (P<0.01), CD4+ (P=0.015) and CD4+ /CD8+ cells (P=0.016) between simple RSV infection and co-infection groups. Conclusions RSV was a common pathogen causing respiratory diseases in children in Hebei Province. The younger the children were, the more likely they were to be infected with RSV. RSV infection was easily complicated by HRV or ADV infection. The epidemic seasons of RSV infection in Hebei were winter and spring. Both simple infection and co-infection of RSV might result in immune dysfunction. Key words: Respiratory syncytial virus; Lymphocyte subsets; Molecular epidemiology; Immunity

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呼吸道合胞病毒致儿童呼吸道感染的流行病学研究及免疫功能变化的初步分析

目的分析河北省儿童呼吸道合胞病毒（RSV）引起的呼吸道疾病的流行病学特点及外周血淋巴细胞亚群和炎症指标的变化。方法收集河北省儿童医院2018年收治的呼吸道感染患儿的9 491份痰标本和9 491对外周血标本。采用多种检测试剂对呼吸道合胞病毒阳性痰标本进行13种呼吸道病原体的筛选。采用流式细胞术检测随机筛选的呼吸道合胞病毒阳性儿童外周血中的T、B淋巴细胞和NK细胞。通过Roche E411发光分析仪测定降钙素原（PCT）。采用罗氏Cobas 8000 C701生化分析仪检测超敏C反应蛋白（hs-CRP）。通过Sysmex XN-BN3血液计测量白细胞（WBC）。结果2018年河北省呼吸道疾病患儿呼吸道合胞病毒阳性率为13.08%。不同年龄组RSV阳性率差异有统计学意义（χ2=479.2976，P＜0.0001）。RSV阳性率随年龄的增长而逐渐下降（χ2=-20.282 7，P＜0.0001），男性高于女性（χ2=34.552 7，P＞0.0001）。呼吸道合胞病毒与其他呼吸道病原体共感染的发生率为29.49%（366/1 241），主要由人鼻病毒（HRV，150/1 241）和腺病毒（ADV，40/1 241）引起。呼吸道合胞病毒感染的主要流行季节为冬季和春季。单纯呼吸道合胞病毒感染和合并感染的流行趋势是一致的。单纯呼吸道合胞病毒感染组和合并感染组的炎症指数、白细胞（P<0.01）、CD4+（P=0.015）和CD4+/CD8+细胞（P=0.016）存在显著差异。结论呼吸道合胞病毒是河北省儿童呼吸道疾病的常见病原。儿童年龄越小，感染呼吸道合胞病毒的可能性就越大。呼吸道合胞病毒感染容易并发HRV或ADV感染。河北省呼吸道合胞病毒感染的流行季节为冬春季。呼吸道合胞病毒的单纯感染和合并感染都可能导致免疫功能障碍。关键词：呼吸道合胞病毒；淋巴细胞亚群；分子流行病学；豁免权

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来源期刊

中华微生物学和免疫学杂志 Immunology and Microbiology-Virology

CiteScore

0.50

自引率

0.00%

发文量

6906

期刊介绍： Chinese Journal of Microbiology and Immunology established in 1981. It is one of the series of journal sponsored by Chinese Medical Association. The aim of this journal is to spread and exchange the scientific achievements and practical experience in order to promote the development of medical microbiology and immunology. Its main contents comprise academic thesis, brief reports, reviews, summaries, news of meetings, book reviews and trends of home and abroad in this field. The distinguishing feature of the journal is to give the priority to the reports on the research of basic theory, and take account of the reports on clinical and practical skills.