Cell pausing method for adipose tissue derived mesenchymal stem cells: comparison of Petaka G3 and ordinary flask

Q1 Biochemistry, Genetics and Molecular Biology

Stem cell investigation Pub Date : 2020-02-19 DOI:10.21037/sci.2019.12.01

J. Pawitan, I. Liem, T. Kispa, Evah Luviah, Fajar Mujadid, Novialdi Novialdi

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引用次数: 0

Abstract

Background: Various methods of cell pausing were applied to prevent proliferation of cultured cells, which either required special medium, using complicated encapsulation method, or using a special flask (Petaka G3 flask). Therefore, this study aimed to develop an easy and economical method of cell pausing method for adipose tissue derived mesenchymal stem cells (AT-MSCs) using ordinary basal medium and flask that yielded AT-MSCs, which were compliant with the requirements of International society for cell therapy (ISCT). Methods: We assessed cell numbers and viabilities, and percentages of CD73, CD90, CD105, and lineage negative, as well as differentiation potentials of AT-MSCs, which were cultured until 80% confluent and then placed at 4 °C or room temperature for three to seven days. We assessed those parameters between fully filled and sealed ordinary flasks compared to Petaka G3 flasks. Results: Cell numbers, viabilities, and percentages of CD73, CD90, CD105, and lineage negative, as well as differentiation potentials of AT-MSCs at room temperature were better than at 4 °C, and at room temperature, results of ordinary flasks were comparable to Petaka G3 flasks. Conclusion: We have developed an easy and economical method of cell pausing method for adipose tissue derived mesenchymal stem cells (AT- MSCs), using ordinary basal medium and flask.

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脂肪组织来源间充质干细胞的细胞暂停法：Petaka G3和普通烧瓶的比较

背景：为了防止培养细胞的增殖，采用了各种细胞暂停方法，这些方法要么需要特殊的培养基，要么使用复杂的包封方法，要么使用特殊的烧瓶（Petaka G3烧瓶）。因此，本研究旨在开发一种简单、经济的脂肪组织来源的间充质干细胞（AT-MSCs）细胞暂停法，使用普通的基础培养基和烧瓶培养AT-MSC，符合国际细胞治疗学会（ISCT）的要求。方法：我们评估了AT-MSCs的细胞数量和存活率、CD73、CD90、CD105和谱系阴性的百分比以及分化潜力，将其培养至80%融合，然后在4°C或室温下放置3至7天。与Petaka G3烧瓶相比，我们在完全填充和密封的普通烧瓶之间评估了这些参数。结果：AT-MSCs在室温下的细胞数量、活性、CD73、CD90、CD105和谱系阴性的百分比以及分化潜能优于4°C，在室温下，普通烧瓶的结果与Petaka G3烧瓶相当。结论：我们开发了一种简单、经济的脂肪组织来源的间充质干细胞（AT-MSCs）细胞暂停法，使用普通基础培养基和烧瓶。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Stem cell investigation Biochemistry, Genetics and Molecular Biology-Developmental Biology

CiteScore

5.80

自引率

0.00%

发文量

期刊介绍： The Stem Cell Investigation (SCI; Stem Cell Investig; Online ISSN: 2313-0792) is a free access, peer-reviewed online journal covering basic, translational, and clinical research on all aspects of stem cells. It publishes original research articles and reviews on embryonic stem cells, induced pluripotent stem cells, adult tissue-specific stem/progenitor cells, cancer stem like cells, stem cell niche, stem cell technology, stem cell based drug discovery, and regenerative medicine. Stem Cell Investigation is indexed in PubMed/PMC since April, 2016.