Conyza blinii responds to the changes of exogenous iron through auxin-terpenoids metabolism pathway

Abstract

ABSTRACT As an essential trace element, iron is a necessary micronutrient for most organisms. Conyza blinii (C. blinii) is a traditional Chinese medicine grown in Sichuan, China. We chose C. blinii as material to explore the way plants respond to iron. Our results showed that iron increased the content of endogenous auxin, glandular trichomes (GTs) density and the content of blinin, the characteristic diterpene in C. blinii. Most of the key enzymes in the blinin synthesis pathway (MEP) were upregulated under iron by RNA sequencing. CbHO-1 (Heme oxygenase gene) was upregulated under exogenous IAA treatment. Taken together, these results suggest that C. blinii respond to iron through auxin- terpenoids metabolism, and this process may be related to CbHO-1.