Differential expression of long non-coding RNAs in peripheral blood of type 2 diabetic patients

Q4 Medicine

中华内分泌代谢杂志 Pub Date : 2019-10-25 DOI:10.3760/CMA.J.ISSN.1000-6699.2019.10.008

Xuan Zhu, Pan Zhang, P. Lou, Yang-guang Du, Ting-jun Liu, C. Qiao, Z. Dong, Peipei Chen, Ting Li

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Abstract

Objective To screen and verify the differential expression profiles of long non-coding RNAs(LncRNAs) in peripheral blood of patients with type 2 diabetes mellitus(T2DM), to identify the potential molecular specific markers of early T2DM. Methods The blood samples of 4 type 2 diabetic patients and 4 normal control subjects were collected for microarray analysis. Then six candidate markers of LncRNAs screened from the differential expression profile were tested by qRT-PCR among the subjects (80 cases in the T2DM group and 50 cases in the control group). The possibility of these LncRNAs as molecular diagnostic markers was analyzed, and finally two of them were carried out by receiver operating characteristic (ROC) analysis. Results Compared with control subjects, there were differentially expressed 133 LncRNAs in type 2 diabetic patients, among which 5 were up-regulated with the maximum up-regulated fold 3.29 and 128 were down-regulated with the maximum down-regulated fold 8.99. Six down-regulated LncRNAs were selected for validation and revealed a similar result to that of microarray.The expressions of two LncRNAs(NONHSAT160746 and NONHSAT140069) in peripheral blood of diabetic patients were significantly lower than those of control subjects (P<0.01). The areas under the ROC curve of the two LncRNAs were 0.734 and 0.703, respectively(P<0.01). Conclusion LncRNAs NONHSAT160746 and LncRNAs NONHSAT140069 are the potential molecular specific markers for the diagnosis of diabetes mellitus. Key words: Diabetes mellitus, type 2; Long non-coding RNAs; Microarray analysis; Biological markers

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长链非编码rna在2型糖尿病患者外周血中的差异表达

目的筛选和验证2型糖尿病(T2DM)患者外周血长链非编码rna (LncRNAs)的差异表达谱，寻找早期T2DM的潜在分子特异性标志物。方法采集4例2型糖尿病患者和4例正常对照者的血液样本进行微阵列分析。然后从差异表达谱中筛选6个候选LncRNAs标记物，通过qRT-PCR在受试者(T2DM组80例，对照组50例)中进行检测。分析了这些lncrna作为分子诊断标记的可能性，最后对其中两个lncrna进行了受试者工作特征(receiver operating characteristic, ROC)分析。结果2型糖尿病患者与对照组相比，差异表达133个lncrna，其中上调5个，最大上调倍数为3.29，下调128个，最大下调倍数为8.99。选择6个下调的lncrna进行验证，结果与微阵列相似。两种lncrna (NONHSAT160746和NONHSAT140069)在糖尿病患者外周血中的表达均显著低于对照组(P<0.01)。两种lncrna的ROC曲线下面积分别为0.734、0.703 (P<0.01)。结论LncRNAs NONHSAT160746和LncRNAs NONHSAT140069是诊断糖尿病的潜在分子特异性标志物。关键词:糖尿病;2型;长链非编码rna;微阵列分析;生物标记物

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来源期刊

中华内分泌代谢杂志 Medicine-Endocrinology, Diabetes and Metabolism

CiteScore

0.60

自引率

0.00%

发文量

7243

期刊介绍： The Chinese Journal of Endocrinology and Metabolism was founded in July 1985. It is a senior academic journal in the field of endocrinology and metabolism sponsored by the Chinese Medical Association. The journal aims to be the "Chinese broadcaster of new knowledge on endocrinology and metabolism worldwide". It reports leading scientific research results and clinical diagnosis and treatment experience in endocrinology and metabolism and related fields, as well as basic theoretical research that has a guiding role in endocrinology and metabolism clinics and is closely integrated with clinics. The journal is a core journal of Chinese science and technology (a statistical source journal of Chinese science and technology papers), and is included in Chinese and foreign statistical source journal databases such as the Chinese Science and Technology Papers and Citation Database, Chemical Abstracts, and Scopus.