Experimental study of 18F-FDG PET/CT imaging and cardiac MRI in diagnosis of radiation-induced myocardial injury in Beagle dogs

中华核医学与分子影像杂志 Pub Date : 2020-01-25 DOI:10.3760/CMA.J.ISSN.2095-2848.2020.01.006

R. Yan, Jian-bo Song, Min Guo, R. Wu, Zhifang Wu, X. Hao, Xiaoshan Guo, Hua Wei, Ping Wu, Li Li, Sijin Li

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Abstract

Objective To investigate the value of 18F-fluorodeoxyglucose (FDG) PET/CT imaging and cardiac MRI (CMR) in the diagnosis of radiation-induced heart disease (RIHD) in Beagle models. Methods Twenty-four normal male Beagle dogs (1-year old) were randomly divided into control group and irradiated groups (3-month, 6-month and 12-month after radiation). The left anterior myocardium of Beagle dogs in irradiated groups was irradiated locally with a single dose of 20 Gy X-ray. Cardiac 18F-FDG PET/CT imaging and CMR were performed on all dogs, and the mean standardized uptake value (SUVmean) and the area of lesions with increased 18F-FDG uptake were obtained. After imaging examinations were finished, dogs were sacrificed and their hearts were taken out to perform Masson staining and electron microcopy. One-way analysis of variance was used for data analysis. Results There was basically no uptake in myocardium in control group. The myocardium showed increased uptake of 18F-FDG in the irradiated groups. The SUVmean of myocardium in 3-month, 6-month and 12-month after radiation groups and control group were 5.90±1.31, 4.66±2.21, 3.21±0.82 and 1.13±0.21, respectively (F=11.81, P<0.05). The area with increased 18F-FDG uptake in the irradiated groups decreased progressively with the prolongation of irradiation time (F=195.74, P<0.01). The reduction in myocardial perfusion and myocardial fibrosis were observed by CMR early at 6-month after irradiation. Compared with the control group, the 6-month and 12-month after radiation groups had increased end diastolic volume (EDV) and end systolic volume (ESV; F=15.479 and 16.908, both P<0.01), and decreased left ventricular ejection fraction (LVEF; F=63.715, P<0.01). The progressive aggravation of myocardial fibrosis was displayed in irradiated groups by Masson staining. The mitochondria degeneration, swelling and the count reduction in irradiated groups were observed by electron microscopy. Conclusions The increased 18F-FDG uptake in the irradiated myocardium may predict the risk of RIHD. 18F-FDG PET/CT imaging can detect RIHD earlier than CMR. Key words: Heart; Radiation injuries, experimental; Positron emission tomography; Tomography, X-ray computed; Deoxyglucose; Magnetic resonance imaging; Dogs

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18F-FDG PET/CT显像和心脏MRI诊断比格犬放射性心肌损伤的实验研究

目的探讨18F-氟脱氧葡萄糖（FDG）PET/CT成像和心脏MRI（CMR）对比格犬模型放射性心脏病（RIHD）的诊断价值。方法24只1岁的正常雄性比格犬随机分为对照组和辐照组（辐照后3个月、6个月和12个月）。用单剂量20Gy X射线局部照射比格犬左前心肌。对所有狗进行心脏18F-FDG PET/CT成像和CMR，获得平均标准化摄取值（SUVmean）和18F-FDG摄取增加的病变面积。影像学检查结束后，处死狗，取出它们的心脏进行Masson染色和电子显微镜检查。数据分析采用单向方差分析。结果对照组心肌基本无摄取。照射组心肌18F-FDG摄取增加。放疗后3个月、6个月和12个月心肌SUV均值分别为5.90±1.31、4.66±2.21、3.21±0.82和1.13±0.21，18F-FDG摄取增加的面积随照射时间的延长而逐渐减小（F=195.74，P<0.01）。CMR在照射后6个月早期观察到心肌灌注和心肌纤维化的减少。与对照组相比，放疗后6个月和12个月组的舒张末期容积（EDV）和收缩末期容积（ESV；F=15.479和16.908，均P<0.01）增加，左心室射血分数（LVEF；F=63.715，P<0.01）降低。电镜观察照射组线粒体变性、肿胀和计数减少。结论放射性心肌18F-FDG摄取增加可预测RIHD的风险。18F-FDG PET/CT成像可以比CMR更早地检测RIHD。关键词：心；辐射损伤，实验性；正电子发射断层扫描；层析成像，X射线计算机；脱氧葡萄糖；磁共振成像；狗

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来源期刊

中华核医学与分子影像杂志核医学，分子影像

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5088

期刊介绍： Chinese Journal of Nuclear Medicine and Molecular Imaging (CJNMMI) was established in 1981, with the name of Chinese Journal of Nuclear Medicine, and renamed in 2012. As the specialized periodical in the domain of nuclear medicine in China, the aim of Chinese Journal of Nuclear Medicine and Molecular Imaging is to develop nuclear medicine sciences, push forward nuclear medicine education and basic construction, foster qualified personnel training and academic exchanges, and popularize related knowledge and raising public awareness. Topics of interest for Chinese Journal of Nuclear Medicine and Molecular Imaging include: -Research and commentary on nuclear medicine and molecular imaging with significant implications for disease diagnosis and treatment -Investigative studies of heart, brain imaging and tumor positioning -Perspectives and reviews on research topics that discuss the implications of findings from the basic science and clinical practice of nuclear medicine and molecular imaging - Nuclear medicine education and personnel training - Topics of interest for nuclear medicine and molecular imaging include subject coverage diseases such as cardiovascular diseases, cancer, Alzheimer’s disease, and Parkinson’s disease, and also radionuclide therapy, radiomics, molecular probes and related translational research.