Antioxidant and anti-inflammatory properties of essential oils from three Eucalyptus species

Q3 Health Professions

Chiang Mai University journal of natural sciences Pub Date : 2021-09-01 DOI:10.12982/cmujns.2021.091

Orapin Insuan, Benchaluk Thongchuai, R. Chaiwongsa, Supaporn Khamchun, W. Insuan

{"title":"Antioxidant and anti-inflammatory properties of essential oils from three Eucalyptus species","authors":"Orapin Insuan, Benchaluk Thongchuai, R. Chaiwongsa, Supaporn Khamchun, W. Insuan","doi":"10.12982/cmujns.2021.091","DOIUrl":null,"url":null,"abstract":"Abstract Eucalyptus essential oils are used as traditional medicines in many countries. The objective of this study was to evaluate the antioxidant and anti-inflammatory activities of leaf essential oils extracted from three different Eucalyptus species on HepG2 and RAW264.7 cells. Essential oils were distilled from fresh leaf samples, and the chemical constituents were analyzed using gas chromatography–mass spectrometry. The antioxidant activities of essential oils were determined using ABTS and hydroxyl radical scavenging assays, and hydrogen peroxide (H2O2)-induced oxidative stress in HepG2 cells. Additionally, lipopolysaccharide (LPS)-activated RAW264.7 macrophages were used to evaluate the anti-inflammatory properties. The results revealed that Eucalyptus citriodora and Eucalyptus urophylla leaf essential oils had a high content of oxygenated monoterpenes, whereas Eucalyptus deglupta contained a high amount of monoterpene hydrocarbons. Essential oils extracted from the three Eucalyptus species showed antioxidant and anti-inflammatory activities. E. citriodora and E. urophylla leaf essential oils had strong antioxidant activity against H2O2-induced oxidative stress in human HepG2 cells. Additionally, E. citriodora leaf essential oil, which contains a high amount of citronellal, exhibited the most potent anti-inflammatory activity in LPS-activated RAW264.7 macrophages. The antioxidant and anti-inflammatory effects of essential oils depended on their chemical composition. A principal component analysis explained 100% of the variance was performed to construct three groups based on the chemical components and antioxidant and anti-inflammatory activities. This study suggests that E. citriodora leaf essential oil, which represents a good source of oxygenated monoterpenes, could be considered a potential phytochemical agent for the prevention of oxidative stress and inflammation. Keywords: Antioxidant, Anti-inflammatory effect, Eucalyptus essential oils, Gas chromatography–mass spectrometry, Principal component analysis","PeriodicalId":10049,"journal":{"name":"Chiang Mai University journal of natural sciences","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chiang Mai University journal of natural sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.12982/cmujns.2021.091","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Health Professions","Score":null,"Total":0}

引用次数: 0

Abstract

Abstract Eucalyptus essential oils are used as traditional medicines in many countries. The objective of this study was to evaluate the antioxidant and anti-inflammatory activities of leaf essential oils extracted from three different Eucalyptus species on HepG2 and RAW264.7 cells. Essential oils were distilled from fresh leaf samples, and the chemical constituents were analyzed using gas chromatography–mass spectrometry. The antioxidant activities of essential oils were determined using ABTS and hydroxyl radical scavenging assays, and hydrogen peroxide (H2O2)-induced oxidative stress in HepG2 cells. Additionally, lipopolysaccharide (LPS)-activated RAW264.7 macrophages were used to evaluate the anti-inflammatory properties. The results revealed that Eucalyptus citriodora and Eucalyptus urophylla leaf essential oils had a high content of oxygenated monoterpenes, whereas Eucalyptus deglupta contained a high amount of monoterpene hydrocarbons. Essential oils extracted from the three Eucalyptus species showed antioxidant and anti-inflammatory activities. E. citriodora and E. urophylla leaf essential oils had strong antioxidant activity against H2O2-induced oxidative stress in human HepG2 cells. Additionally, E. citriodora leaf essential oil, which contains a high amount of citronellal, exhibited the most potent anti-inflammatory activity in LPS-activated RAW264.7 macrophages. The antioxidant and anti-inflammatory effects of essential oils depended on their chemical composition. A principal component analysis explained 100% of the variance was performed to construct three groups based on the chemical components and antioxidant and anti-inflammatory activities. This study suggests that E. citriodora leaf essential oil, which represents a good source of oxygenated monoterpenes, could be considered a potential phytochemical agent for the prevention of oxidative stress and inflammation. Keywords: Antioxidant, Anti-inflammatory effect, Eucalyptus essential oils, Gas chromatography–mass spectrometry, Principal component analysis

查看原文本刊更多论文

三种桉树精油的抗氧化和抗炎特性

摘要桉树精油在许多国家被用作传统药物。本研究的目的是评估从三种不同桉树中提取的叶精油对HepG2和RAW264.7细胞的抗氧化和抗炎活性。从新鲜叶片样品中蒸馏精油，并使用气相色谱-质谱法分析其化学成分。使用ABTS和羟基自由基清除法测定精油的抗氧化活性，以及过氧化氢（H2O2）诱导的HepG2细胞氧化应激。此外，脂多糖（LPS）激活的RAW264.7巨噬细胞用于评估抗炎特性。结果表明，柠檬桉和尾叶桉叶精油中含氧单萜烯含量较高，而德格卢塔桉中含大量单萜烯烃。从三种桉树中提取的精油具有抗氧化和抗炎活性。柠檬叶和尾叶精油对H2O2诱导的人HepG2细胞氧化应激具有较强的抗氧化活性。此外，含有大量香茅醛的柠檬叶精油在LPS激活的RAW264.7巨噬细胞中表现出最有效的抗炎活性。精油的抗氧化和抗炎作用取决于它们的化学成分。根据化学成分、抗氧化和抗炎活性，进行了100%方差的主成分分析，构建了三组。这项研究表明，柠檬叶精油是含氧单萜的良好来源，可以被认为是预防氧化应激和炎症的潜在植物化学剂。关键词：抗氧化剂，抗炎作用，桉树精油，气相色谱-质谱法，主成分分析

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Chiang Mai University journal of natural sciences Health Professions-Health Professions (miscellaneous)

CiteScore

1.70

自引率

0.00%

发文量