Antibacterial Activity of Origanum vulgare and Rosmarinus officinalis standardized extracts Against Curtobacterium and Xanthomonas

Q4 Social Sciences

Fronteiras Pub Date : 2023-04-28 DOI:10.21664/2238-8869.2023v12i1.p110-122

P. Vaz, Waléria Ramos Nogueira de Souza, A. Wendland, M. G. D. Oliveira, Hérica Núbia Cardoso Cirilo, M. V. Cortes, M. Bara

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引用次数: 0

Abstract

Diseases caused by Curtobacterium and Xantomonnas species represent an agricultural problem in crops and can generate economic impacts on the commercialization of seeds and food. Origanum vulgare L. (oregano) and Rosmarinus officinalis L. (rosemary) have rosmarinic acid and others phenolics that can lead to the control of phytopathogenic bacteria in common bean (Phaseolus vulgaris L.). This study aimed to evaluate the in vitro and in vivo antibacterial activity of O. vulgaris and R. officinalis extracts, standardized in rosmarinic acid, against Curtobacterium flaccumfaciens pv. flaccumfaciens, Xanthomonas axonopodis pv. phaseoli, Xanthomonas fuscans subsp. fuscans and Xanthomonas sp. The antibacterial effect of the extracts in bean seed was also investigated. The content of rosmarinic acid was 8.55 % for O. vulgare and 16.30 % for R. officinalis extract. It was verified the complete in vitro inhibition of the bacteria studied by both extracts at 0.8% (w/w) with exception of Xanthomonas axonopodis pv. phaseoli BRM 025302 that was completely inhibited at 1.2% (w/w) of oregano. In addition, no symptom of phytotoxicity were noted in detached bean leaves treated with them. Under greenhouse conditions, some reduction on severity of Curtobacterium wilt by both extracts at 1% (w/w) was noted to bean cultivars BRS Sublime and BRS Estilo. Under the experimental conditions these extracts were not efficient to control the common bacterial blight caused by X. axonopodis pv. phaseoli. Both extracts are promising in the treatment of seeds, specially in related to contamination by Fusarium spp., whose percentage decreased on average an average from 94% to 10%. In addition, these bean seeds maintained the germination percentage adequate to that required by legislation. Further studies must be conducted to better investigate the potential of these standardized extracts as a bioproduct for agriculture.

查看原文本刊更多论文

土茯苓和迷迭香标准提取物对Curtobacterium和Xanthomonas的抑菌活性

由枯杆菌和黄单胞菌引起的疾病是农作物的一个农业问题，可能对种子和粮食的商业化产生经济影响。牛至(Origanum vulgare L.)和迷迭香(Rosmarinus officinalis L.)含有迷迭香酸和其他酚类物质，可以控制普通豆(Phaseolus vulgaris L.)中的植物致病菌。本研究旨在评价迷迭香酸标配的寻常花和officinalis提取物对flaccumfaciens pv的体外和体内抑菌活性。黄单胞菌;黄单胞菌fuscan亚种。研究了豆籽提取物的抑菌作用。香姜提取物中迷迭香酸含量分别为8.55%和16.30%。结果表明，除axonopodxanthomonas pv外，两种提取物的体外抑菌率均为0.8% (w/w)。在牛至含量为1.2% (w/w)时，phaseoli BRM 025302被完全抑制。此外，用它们处理的离体豆叶没有出现植物毒性症状。在温室条件下，这两种提取物在1% (w/w)浓度下对大豆品种BRS Sublime和BRS Estilo的枯萎病严重程度均有一定的降低。在实验条件下，这些提取物对普通细菌性叶枯病的防治效果不明显。phaseoli。两种提取物在处理种子中均有较好的应用前景，特别是在处理镰刀菌污染方面，其处理比例从94%下降到10%。此外，这些豆种子保持了足够的发芽率，符合法律要求。必须进行进一步的研究，以更好地调查这些标准化提取物作为农业生物产品的潜力。

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来源期刊

Fronteiras Social Sciences-Social Sciences (all)

CiteScore

0.40

自引率

0.00%

发文量

审稿时长

10 weeks

期刊介绍： FRONTEIRAS: JOURNAL OF SOCIAL, TECHNOLOGICAL AND ENVIRONMENTAL SCIENCE is an open-access, quarterly, peer-reviewed publication maintained by the Graduate Studies in Social, Technological and Environmental Science of the University Center of Anápolis (PPG STMA), with masters and doctoral degrees in Environmental Sciences. Fronteiras is an interdisciplinary journal that is driven by the belief that all types of knowledge must be available. It has as a target the researchers who dialogue in an interdisciplinary way with the theme "social and environmental science". The journal aims to publish original research, recognized rigorously for its theoretical-methodological, intellectual and scientific relevance. Fronteira has as the main theme of its publications the environmental science, aiming the scientific dissemination arising from the dialogues between the natural and human sciences and their connections with environmental science. Fronteiras: Journal of Social, Technological and Environmental Science has as its mission to publish and disseminate among the academic community, scientific manuscripts that are original and contribute to the promotion of multidisciplinary dialogues between the natural and human sciences and their connections with technological innovation, health as well as the environment.