Polymer-based Electrochemical Sensor: Fast, Accurate, and Simple Insulin Diagnostics Tool

IF 2.7 4区化学 Q3 CHEMISTRY, PHYSICAL

Electrocatalysis Pub Date : 2023-05-10 DOI:10.1007/s12678-023-00827-w

Ivana Šišoláková, Radka Gorejová, Frederika Chovancová, Jana Shepa, Fahanwi Asabuwa Ngwabebhoh, Andrea Straková Fedorková, Petr Sáha, Renáta Oriňaková

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Abstract

Study of the use of polymers with higher conductivity like polypyrrole, and polyaniline in the electrochemical insulin sensors can overcome the drawbacks arising from the ongoing use of non-conductive polymer membrane. Conductive polymer membranes maintain the positive properties of polymers, like improved stability, reproducibility, and even increase the current response of the prepared sensor toward insulin oxidation. Three different screen-printed electrodes modified with polyaniline, polypyrrole, or chitosan with electrochemically deposited nickel nanoparticles ensuring insulin oxidation were prepared. The electrode morphology was examined via SEM with EDX analysis. Also, the electroactive surface area and stability were determined by voltammetric methods. Based on the results, the SPCEs modified by polypyrrole and nickel nanoparticles were determined as the most appropriate for the insulin determination. The NiNPs-PPy-SPCE exhibited a linear range (500 nM–5 µM), a low-down limit of detection (38 nM), high sensitivity (3.98 µA/µM), and excellent result from insulin determination in real samples (human blood serum). The results confirmed the high potential of developed sensor for future research focused on detection of insulin via electrochemistry methods in clinical samples.

Graphical Abstract

Abstract Image

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基于聚合物的电化学传感器:快速，准确，简单的胰岛素诊断工具

研究在电化学胰岛素传感器中使用诸如聚吡咯、聚苯胺等电导率较高的聚合物，可以克服目前使用非导电聚合物膜所带来的缺点。导电聚合物膜保持了聚合物的积极特性，如提高了稳定性、可重复性，甚至增加了所制备的传感器对胰岛素氧化的电流响应。制备了三种不同的丝网印刷电极，分别用聚苯胺、聚吡咯或壳聚糖修饰，并以电化学沉积的纳米镍保证胰岛素氧化。通过扫描电镜和EDX分析检测电极形貌。用伏安法测定了其电活性表面积和稳定性。结果表明，聚吡咯和纳米镍修饰的spce最适合用于胰岛素的检测。NiNPs-PPy-SPCE具有良好的线性范围(500 nM - 5µM)、下限(38 nM)、高灵敏度(3.98µa /µM)和对真实样品(人血清)胰岛素的检测效果。结果证实了该传感器在未来的研究中具有很高的潜力，可以通过电化学方法在临床样品中检测胰岛素。图形抽象

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来源期刊

Electrocatalysis CHEMISTRY, PHYSICAL-ELECTROCHEMISTRY

CiteScore

4.80

自引率

6.50%

发文量

审稿时长

>12 weeks

期刊介绍： Electrocatalysis is cross-disciplinary in nature, and attracts the interest of chemists, physicists, biochemists, surface and materials scientists, and engineers. Electrocatalysis provides the unique international forum solely dedicated to the exchange of novel ideas in electrocatalysis for academic, government, and industrial researchers. Quick publication of new results, concepts, and inventions made involving Electrocatalysis stimulates scientific discoveries and breakthroughs, promotes the scientific and engineering concepts that are critical to the development of novel electrochemical technologies. Electrocatalysis publishes original submissions in the form of letters, research papers, review articles, book reviews, and educational papers. Letters are preliminary reports that communicate new and important findings. Regular research papers are complete reports of new results, and their analysis and discussion. Review articles critically and constructively examine development in areas of electrocatalysis that are of broad interest and importance. Educational papers discuss important concepts whose understanding is vital to advances in theoretical and experimental aspects of electrochemical reactions.