Association of Two MMP-8 Gene Polymorphisms With Recurrent Pregnancy Loss in Iranian Women

Abstract

Background: It has been reported that less than 5% of women experience recurrent pregnancy loss (RPL). different matrix metalloproteinases (MMPs) have proteolysis function with a main role in the stable development of the fetus. Objective: This study aims to assess the associations between two single nucleotide polymorphisms (rs2509013 C>T and rs11225395 G>A) of MMP-8 gene and RPL among 130 Iranian women with a history of RPL and 130 controls. Methods: Genotyping of the MMP-8 gene was done for the two polymorphisms by using Sanger sequencing method. Results: High frequency of AA genotype (OR: 2.5, 95%CI:1.02-4.1, P<0.01) and A allele (OR:1.95, 95% CI:0.95-3.1, P<0.001) of rs11225395 G>A polymorphism in patients compared to controls. This high frequency was also reported in the haplotypes and combined genotypes of polymorphism. Conclusion: The MMP-8 gene may be involved in RPL risk and is a potential biomarker for RPL susceptibility.